Related Experiment Video
Updated: May 27, 2026

Species Determination and Quantitation in Mixtures Using MRM Mass Spectrometry of Peptides Applied to Meat Authentication
Published on: September 20, 2016
Analytical methods for authentication of fresh vs. thawed meat - A review
1Department of Food Chemistry, Regional Veterinary and Food Control Authority, Danish Veterinary and Food Administration, Soendervang 4, DK-4100 Ringsted, Denmark; Department of Food Science, University of Copenhagen, Rolighedsvej 30, DK-1958 Frederiksberg C, Denmark.
Mislabeling of thawed meat as fresh meat is common, affecting 8-15% of samples. A combination of analytical methods is best for detecting thawed meat and determining frozen storage time in beef, pork, and chicken.
Area of Science:
- Food Science
- Analytical Chemistry
- Meat Science
Background:
- Accurate meat product labeling is crucial for consumer trust and fair trade.
- Prevalence of mislabeled meat (thawed sold as fresh) ranges from 8-15% in analyzed samples.
- Regulatory enforcement necessitates reliable analytical methods for meat authentication.
Purpose of the Study:
- To review analytical techniques for distinguishing fresh from thawed meat.
- To assess methods for determining frozen storage duration.
- To identify optimal analytical strategies for meat traceability.
Main Methods:
- Review of enzymatic, DNA-based, spectroscopic, bio-imaging, and sensory techniques.
- Analysis of molecular changes detected by various methods.
- Evaluation of method capabilities for fresh vs. thawed meat discrimination and frozen storage time determination.
Main Results:
- Various analytical techniques detect molecular changes indicative of thawing and frozen storage.
- Enzymatic, DNA-based, spectroscopic, bio-imaging, and sensory methods show varying capabilities.
- Discrimination between fresh and thawed meat and determination of frozen storage time are achievable with these techniques.
Conclusions:
- A combination of analytical methods provides the most effective approach for discriminating fresh from thawed meat.
- Accurate identification of meat origin and processing history is vital.
- Further research into combined analytical strategies will enhance meat authenticity verification.
Related Concept Videos
Methods of Controlling Food Spoilage
Methods of Classification and Identification
Physical Methods for Controlling Microbial Growth: Temperature

