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[Titration and stability tests for antimeasles vaccine]
1Departamento de Vacunas Virales, Laboratorio Nacional de Salud Pública, México, D.F.
Abstract:
Production and quality of the vaccines, are important activities in the prevention of measles disease. Edmonston-Zagreb measles vaccine is the main strain attenuated virus distributed in México. In order to evaluate the titer of Edmonston-Zagreb and Schwarz vaccines, 50% tissue culture infective dose (TCID50/0.5 mL) and plaque-forming units (PFU) are performed in the National Laboratory of Public Health. Furthermore PFU is a method used in the stability vaccine test. Validation criteria are described in this paper.
Insights
High-quality measles vaccines are vital for disease prevention. This study evaluates Edmonston-Zagreb and Schwarz vaccine titers using TCID50 and plaque-forming units (PFU) methods.
Area of Science:
- Virology
- Vaccinology
- Public Health
Context:
- Measles prevention relies on effective vaccine production and quality control.
- The Edmonston-Zagreb strain is the primary live-attenuated measles virus vaccine used in Mexico.
- Assessing vaccine potency is crucial for ensuring public health initiatives.
Purpose:
- To evaluate the titer of Edmonston-Zagreb and Schwarz measles vaccines.
- To detail the methodology for vaccine titer determination using 50% tissue culture infective dose (TCID50/0.5 mL) and plaque-forming units (PFU).
- To describe the validation criteria for these analytical methods.
Summary:
- The study assessed measles vaccine titers in Mexico, comparing Edmonston-Zagreb and Schwarz strains.
- Standard methods, including TCID50 and PFU, were employed for quantitative analysis.
- PFU was also utilized for vaccine stability testing, with validation criteria outlined.
Impact:
- This research provides essential data on measles vaccine quality in Mexico.
- The described methodologies and validation criteria can inform vaccine quality control standards.
- Ensuring accurate vaccine titration supports the efficacy of measles disease prevention programs.