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Related Concept Videos

Ribosomal RNA Synthesis02:53

Ribosomal RNA Synthesis

Ribosome synthesis is a highly complex and coordinated process involving more than 200 assembly factors. The synthesis and processing of ribosomal components occurs not only in the nucleolus but also in the nucleoplasm and the cytoplasm of eukaryotic cells.
Ribosome biogenesis begins with the synthesis of 5S and 45S pre-rRNAs by distinct RNA polymerases. The primary transcripts are extensively processed and modified before they are bound and folded by ribosomal proteins and assembly factors,...
Ribosomal RNA Synthesis02:53

Ribosomal RNA Synthesis

Ribosome synthesis is a highly complex and coordinated process involving more than 200 assembly factors. The synthesis and processing of ribosomal components occurs not only in the nucleolus but also in the nucleoplasm and the cytoplasm of eukaryotic cells.
Ribosome biogenesis begins with the synthesis of 5S and 45S pre-rRNAs by distinct RNA polymerases. The primary transcripts are extensively processed and modified before they are bound and folded by ribosomal proteins and assembly factors,...
Ribosomes01:27

Ribosomes

Ribosomes translate genetic information encoded by messenger RNA (mRNA) into proteins. Both prokaryotic and eukaryotic cells have ribosomes. Cells that synthesize large quantities of protein—such as secretory cells in the human pancreas—can contain millions of ribosomes.
Ribosome Structure and Assembly
Ribosomes are composed of ribosomal RNA (rRNA) and proteins. In eukaryotes, rRNA is transcribed from genes in the nucleolus—a part of the nucleus that specializes in ribosome production. Within...
Ribosomes01:27

Ribosomes

Ribosomes translate genetic information encoded by messenger RNA (mRNA) into proteins. Both prokaryotic and eukaryotic cells have ribosomes. Cells that synthesize large quantities of protein—such as secretory cells in the human pancreas—can contain millions of ribosomes.
Ribosome Structure and Assembly
Ribosomes are composed of ribosomal RNA (rRNA) and proteins. In eukaryotes, rRNA is transcribed from genes in the nucleolus—a part of the nucleus that specializes in ribosome production. Within...
Ribosomes01:27

Ribosomes

Ribosomes translate genetic information encoded by messenger RNA (mRNA) into proteins. Both prokaryotic and eukaryotic cells have ribosomes. Cells that synthesize large quantities of protein—such as secretory cells in the human pancreas—can contain millions of ribosomes.
Ribosome Structure and Assembly
Ribosomes are composed of ribosomal RNA (rRNA) and proteins. In eukaryotes, rRNA is transcribed from genes in the nucleolus—a part of the nucleus that specializes in ribosome production. Within...
Ribosomes01:27

Ribosomes

Ribosomes translate genetic information encoded by messenger RNA (mRNA) into proteins. Both prokaryotic and eukaryotic cells have ribosomes. Cells that synthesize large quantities of protein—such as secretory cells in the human pancreas—can contain millions of ribosomes.
Ribosome Structure and Assembly
Ribosomes are composed of ribosomal RNA (rRNA) and proteins. In eukaryotes, rRNA is transcribed from genes in the nucleolus—a part of the nucleus that specializes in ribosome production. Within...

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Related Experiment Video

Updated: May 27, 2026

Eukaryotic Polyribosome Profile Analysis
09:16

Eukaryotic Polyribosome Profile Analysis

Published on: June 15, 2010

SUMO routes ribosome maturation.

Elisabeth Finkbeiner1, Markus Haindl, Nithya Raman

  • 1Department of Molecular Cell Biology, Max Planck Institute of Biochemistry, Martinsried, Germany.

Nucleus (Austin, Tex.)
|November 9, 2011
PubMed
Summary

Ribosome biogenesis control is vital for cell growth. SUMOylation of PELP1 by SENP3 regulates the PELP1-TEX10-WDR18 complex, ensuring proper 60S subunit maturation and nucleolar-nucleoplasmic transit.

Area of Science:

  • Cell Biology
  • Molecular Biology
  • Biochemistry

Background:

  • Ribosome biogenesis is crucial for protein synthesis, cell growth, and proliferation.
  • Mammalian ribosome subunit maturation involves complex pathways in the nucleolus and nucleoplasm.
  • Post-translational modifications, particularly SUMOylation, are increasingly recognized as key regulators.

Purpose of the Study:

  • To identify novel targets of the SUMO-specific isopeptidase SENP3.
  • To elucidate the role of SENP3 in the 60S ribosomal subunit maturation pathway.
  • To investigate the function of the PELP1-TEX10-WDR18 complex in ribosome biogenesis.

Main Methods:

  • Co-immunoprecipitation to identify protein complexes.
  • SUMOylation assays to detect post-translational modifications.

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RIBO-seq in Bacteria: a Sample Collection and Library Preparation Protocol for NGS Sequencing

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Genome-wide Quantification of Translation in Budding Yeast by Ribosome Profiling
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Genome-wide Quantification of Translation in Budding Yeast by Ribosome Profiling

Published on: December 21, 2017

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Published on: June 15, 2010

RIBO-seq in Bacteria: a Sample Collection and Library Preparation Protocol for NGS Sequencing
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RIBO-seq in Bacteria: a Sample Collection and Library Preparation Protocol for NGS Sequencing

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Genome-wide Quantification of Translation in Budding Yeast by Ribosome Profiling

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  • Microscopy and cell fractionation to track protein localization and complex assembly.

    • Main Results:

    • A novel SENP3-associated complex containing PELP1, TEX10, and WDR18 was identified.
    • This complex is involved in the nucleolar maturation and nucleoplasmic transit of the 60S ribosomal subunit.
    • PELP1 is a SUMOylated target of SENP3, and its desumoylation is essential for the complex's nucleolar localization.

    Conclusions:

    • SENP3-mediated desumoylation of PELP1 is critical for the correct subnuclear trafficking of the PELP1-TEX10-WDR18 complex.
    • SUMOylation of PELP1 may act as a quality control mechanism, preventing premature loading onto 60S particles.
    • This SUMO-dependent regulation contributes to coordinating ribosome formation with cellular needs.