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Updated: May 27, 2026
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Isolation & Characterization of Hoechstlow CD45negative Mouse Lung Mesenchymal Stem Cells
Published on: October 26, 2011
Isolation & characterization of Hoechst(low) CD45(negative) mouse lung mesenchymal stem cells
Kelsey S Chow1, DuHyun Jun, Karen M Helm
1Charles C. Gates Regenerative Medicine and Stem Cell Biology Program, University of Colorado Denver, USA.
Journal of Visualized Experiments : Jove
|November 9, 2011
Summary
Resident lung mesenchymal stem cells (MSCs) regulate tissue repair and disease. This study defines a method to isolate and study these crucial cells in pulmonary tissue.
Area of Science:
- Pulmonary Medicine
- Stem Cell Biology
- Regenerative Medicine
Background:
- Mesenchymal stem cells (MSCs) are vital for tissue repair, regeneration, and disease processes.
- Resident lung MSCs are implicated in pulmonary homeostasis, injury, and diseases like pulmonary fibrosis (PF) and pulmonary arterial hypertension (PAH).
Purpose of the Study:
- To develop and describe a technology for defining and isolating resident lung MSCs in vivo.
- To enable the study of specific lung MSC populations and their functions in pulmonary health and disease.
Main Methods:
- Utilizing a defined set of cell surface markers to identify resident lung MSCs within pulmonary tissue.
- Employing flow cytometry for the repeated isolation of a well-characterized MSC population.
Main Results:
- Successful definition of a specific population of resident lung MSCs in vivo.
- Establishment of a reproducible method for isolating these cells using flow cytometry.
Conclusions:
- The described technology allows for the precise identification and isolation of resident lung MSCs.
- This facilitates further research into the role of lung MSCs in pulmonary tissue homeostasis and disease pathogenesis.
