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An Aquatic Microbial Metaproteomics Workflow: From Cells to Tryptic Peptides Suitable for Tandem Mass Spectrometry-based Analysis
Published on: September 15, 2015
Proteome profiling of the green sulfur bacterium Chlorobaculum tepidum by N-terminal proteomics
Kalliopi Kouyianou1, Pieter-Jan De Bock, Niklaas Colaert
1Division of Biochemistry, Department of Chemistry, University of Crete, Voutes Heraklion, Greece.
Abstract:
In this study, we performed the first large-scale identification of N-terminal peptides from the green sulfur bacterium Chlorobaculum tepidum. Combined fractional diagonal chromatography (COFRADIC) was used to isolate protein N-terminal peptides from three different proteome preparations, and following LC-MS/MS analysis, over 621 different proteins were identified by their N-terminal peptides. Our data constitute the largest data set currently available for protein N-termini of prokaryotic photosynthetic organisms.
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