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Yeast As a Chassis for Developing Functional Assays to Study Human P53
Published on: August 4, 2019
Polymorphisms in apoptosis-related genes and TP53 mutations in non-small cell lung cancer
Eun Young Bae1, Eun Jin Lee, Hyo-Gyoung Kang
1Department of Biochemistry and Cell Biology, Kyungpook National University School of Medicine, Daegu, Korea.
Abstract:
Apoptosis plays an essential role in the elimination of mutated or transformed cells from the body. Therefore, polymorphisms of apoptosis-related genes may lead to an alteration in apoptotic capacity, thereby affecting the occurrence of TP53 mutations in lung cancer. We investigated the relationship between potentially functional polymorphisms of apoptosis-related genes and TP53 mutations in non-small cell lung cancer (NSCLC). Twenty-seven single nucleotide polymorphisms in 20 apoptosis-related genes were genotyped by a sequenome mass spectrometry-based genotyping assay in 173 NSCLCs and the associations with TP53 mutations in the entire coding exons (exons 2-11), including splicing sites of the gene, were analyzed. None of the 27 polymorphisms was significantly associated with the occurrence of TP53 mutations. This suggests that apoptosis-related genes may not play an important role in the occurrence of TP53 mutations in lung cancer.
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