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Updated: May 27, 2026
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Published on: August 23, 2024
[Optimization of microwave-assisted extraction with RSM and HPLC determination for three iridoid glycosides in Cornus
Juan-ni He1, Xiao Liu, Zhao-li Mou
1Key Laboratory of Medicinal Resource and Natural Pharmaceutical Chemistry of Ministry of Education, School of Chemistry and Material, Shaanxi Normal University, Xi'an 710062, China. hjn2008@stu.snnu.edu.cn
Objective:
To establish a method of microwave-assisted extraction and high performance liquid chromatographic (HPLC) for simultaneous determination of three iridoid glycosides including loganin, sweroside and cornuside in Cornus officinalis.
Methods:
The extraction conditions of microwave power,ethanol concentration, liquid to sample ratio were optimized with a response surface methodology (RSM); Three constituents were separated on an Agilent TC-C18 column by gradient elution using acetonitrile and water as the mobile phase. The flow rate was 1.0 mL/min. The detection wave length was 240 nm.
Results:
The optimal conditions of microwave extraction were as follows: microwave power 400 W, ethanol concentration 72%, liquid to sample ratio 15 mL/g, the extraction time 10 min, the extraction times 2; The HPLC peak areas of all the constituents showed good linearity (r>0.9994) in the range of the tested concentration,the average recoveries of the method were 98.68%, 98.24% and 98.29%, respectively.
Conclusions:
The established method of microwave-assisted extraction and HPLC simultaneously determination has the advantages of convenient, precision and reliability. It can be used in simultaneous determination of three iridoid glycosides in Cornus officinalis.