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Updated: May 27, 2026
GC-based Detection of Aldononitrile Acetate Derivatized Glucosamine and Muramic Acid for Microbial Residue Determination in Soil
Published on: May 19, 2012
[Determination of underivatized glycine and proline in vinegar turtle shell by HPLC-ELSD]
Xinyu Wang1, Xiaomei Tan, Mingze Gao
1Key Laboratory of Traditional Chinese Medicine Preparation of Guangdong Province, School of Traditional Chinese Medicine, Southern Medical University, Guangzhou 510515, China. wangxy1225@126.com
Objective:
To establish a method to determine the underivatized glycine (Gly) and proline (Pro) in vinegar turtle shell.
Method:
An HPLC-ELSD method was conducted on a Prevail C18 column (4.6 mm x 250 mm, 5 microm) with the mobile phase of acetonitrile and 0.7% trifluoroacetic acid solution (containing 5.0 mmol x L(-1) heptafluorobutyric acid), and elution time was 15 min.
Result:
The calibration curves were showed good linearity within the concentration range of 0.14-0.6 g x L(-1). The average recoveries were 101.2% and 102.5%, and RSD were 1.9% and 2.5%, respectively.
Conclusion:
Since this method needs neither the special amino acid analyzer nor derivation of amino acid., it is efficient, simple and accurate., which could be used for quality control of vinegar turtle shell.
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