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A new assay for endonuclease/methyltransferase activities based on graphene oxide

Jieon Lee1, Young-Kwan Kim, Dal-Hee Min

  • 1Department of Chemistry, KAIST, Daejeon, 305-701, Republic of Korea.

Analytical Chemistry
|November 10, 2011
PubMed

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Restriction enzymes are bacterial enzymes used to cut DNA in a sequence-specific manner. To cleave DNA, they bind to specific palindromic sequences called restriction sites. Such palindromic DNA sequences or inverted repeats are commonly found in regions of functional significance, such as the origin of replication, gene operator sites, and regions containing transcription termination signals.
The host bacteria protect their own genomic DNA from these enzymes by methylating these sites. Some...