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Updated: May 27, 2026

Development and Functionalization of Electrolyte-Gated Graphene Field-Effect Transistor for Biomarker Detection
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Development and Functionalization of Electrolyte-Gated Graphene Field-Effect Transistor for Biomarker Detection

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Impedimetric thrombin aptasensor based on chemically modified graphenes.

Adeline Huiling Loo1, Alessandra Bonanni, Martin Pumera

  • 1Division of Chemistry & Biological Chemistry, School of Physical and Mathematical Sciences, Nanyang Technological University, Singapore 637371, Singapore.

Nanoscale
|November 10, 2011
PubMed
Summary
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Graphene oxide demonstrates superior performance for a label-free electrochemical aptasensor detecting thrombin. This biosensor offers a simple, sensitive, and selective method for biomarker detection in clinical diagnostics.

Area of Science:

  • Biomedical Engineering
  • Materials Science
  • Analytical Chemistry

Background:

  • Highly sensitive biosensors are crucial for biomedical applications.
  • Graphene is a promising material for biosensor development due to its unique properties.
  • Various chemically modified graphene (CMG) materials exist, each with potential for biosensing.

Purpose of the Study:

  • To compare the biosensing performance of different chemically modified graphene (CMG) platforms.
  • To develop a simple, label-free electrochemical impedimetric aptasensor for thrombin detection.
  • To identify the optimal CMG material for aptasensor fabrication.

Main Methods:

  • Fabrication of disposable screen-printed electrodes modified with graphite oxide (GPO), graphene oxide (GO), thermally reduced graphene oxide (TR-GO), and electrochemically reduced graphene oxide (ER-GO).

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Published on: March 31, 2022

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Last Updated: May 27, 2026

Development and Functionalization of Electrolyte-Gated Graphene Field-Effect Transistor for Biomarker Detection
07:51

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Exploring Biomolecular Interaction Between the Molecular Chaperone Hsp90 and Its Client Protein Kinase Cdc37 using Field-Effect Biosensing Technology
09:39

Exploring Biomolecular Interaction Between the Molecular Chaperone Hsp90 and Its Client Protein Kinase Cdc37 using Field-Effect Biosensing Technology

Published on: March 31, 2022

  • Immobilization of a DNA aptamer specific to thrombin onto the modified electrodes.
  • Electrochemical impedance spectroscopy (EIS) for label-free detection of thrombin binding to the aptamer.

    • Main Results:

    • Graphene oxide (GO) exhibited the best performance among the tested CMG materials.
    • The optimal aptamer concentration was determined to be 10 μM.
    • The aptasensor demonstrated a linear detection range for thrombin from 10-50 nM with high selectivity.

    Conclusions:

    • Graphene oxide is the most suitable material for developing simple, label-free electrochemical aptasensors.
    • The developed GO-based aptasensor shows significant promise for clinical diagnosis and point-of-care analysis of biomarkers.
    • This study provides valuable insights into selecting appropriate graphene materials for advanced biosensor applications.