Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Differentiation between leaks and slips in oxidative phosphorylation.

B H Groen1, J A Berden, K van Dam

  • 1E.C. Slater Institute for Biochemical Research, University of Amsterdam, The Netherlands.

Biochimica Et Biophysica Acta
|August 30, 1990
PubMed
Summary

Researchers measured the thermodynamic efficiency of oxidative phosphorylation in rat liver mitochondria. They distinguished between proton leak and redox slip effects on energy transduction, finding experimental results matched predictions.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Factors associated with the onset of major depressive disorder in adults with type 2 diabetes living in 12 different countries: results from the INTERPRET-DD prospective study.

Epidemiology and psychiatric sciences·2020
Same author

Longitudinal associations between depression and diabetes complications: a systematic review and meta-analysis.

Diabetic medicine : a journal of the British Diabetic Association·2019
Same author

Personality dimensions and their relationship with job satisfaction amongst Dutch nurse anaesthetists.

Journal of nursing management·2010
Same author

Job satisfaction amongst Dutch nurse anaesthetists: the influence of emotions on events.

International nursing review·2010
Same author

Burnout, psychosomatic symptoms and job satisfaction among Dutch nurse anaesthetists: a survey.

Acta anaesthesiologica Scandinavica·2010
Same author

The impact of social support upon intention to leave among female nurses in Europe: secondary analysis of data from the NEXT survey.

International journal of nursing studies·2009

Area of Science:

  • Mitochondrial bioenergetics
  • Cellular respiration
  • Biochemical thermodynamics

Background:

  • Oxidative phosphorylation is the primary energy-generating process in aerobic cells.
  • Proton leak and redox slip are potential inefficiencies in oxidative phosphorylation.
  • Understanding these inefficiencies is crucial for comprehending cellular energy transduction.

Purpose of the Study:

  • To measure the thermodynamic efficiency of oxidative phosphorylation in isolated rat-liver mitochondria.
  • To theoretically calculate the impact of proton leak and redox slip on energy transduction efficiency.
  • To experimentally validate the theoretical predictions by inducing proton leak or redox slip.

Main Methods:

  • Measurement of thermodynamic efficiency during succinate oxidation in isolated rat-liver mitochondria.

Related Experiment Videos

  • Computational modeling to predict the effects of proton leak and redox slip.
  • Experimental induction of proton leak and redox slip to assess their impact on efficiency.

    • Main Results:

    • Experimental measurements of mitochondrial efficiency were obtained.
    • Calculated effects of proton leak and redox slip on energy transduction efficiency were determined.
    • Experimental results aligned with theoretical predictions, confirming the models.

    Conclusions:

    • Proton leak and redox slip have distinct effects on energy transduction efficiency.
    • It is experimentally feasible to differentiate between the impacts of proton leak and redox slip.
    • This study provides a framework for analyzing inefficiencies in mitochondrial energy production.