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Related Concept Videos

Immunoprecipitation01:20

Immunoprecipitation

Immunoprecipitation, or IP, is a widely used technique that employs protein-antibody interactions to isolate proteins or protein complexes in their native state for studying protein-protein interactions, quaternary structures, or supramolecular complexes. Various modifications of the technique, including chromatin IP, cross-linking IP, and fluorescence IP, are commonly used.
Chromatin Immunoprecipitation
Chromatin immunoprecipitation, also known as ChIP, is used to study protein-DNA or...
Hybridoma Technology01:31

Hybridoma Technology

Hybridoma technology is used for the large-scale production of monoclonal antibodies. Monoclonal antibodies bind to only a single antigenic determinant or epitope. Such antibodies are used in research, diagnostics, and disease therapy. The hybridoma technology established in 1975 by Georges Köhler and Cesar Milstein was awarded the Nobel Prize in Medicine in 1984 for revolutionizing research and therapy.
Hybridoma Selection
Commonly used fusion techniques — electroporation, polyethylene glycol...
Types Of Column Chromatography01:29

Types Of Column Chromatography

The stability and compatibility of column material with samples are crucial for efficient purification in chromatographic techniques. Various operating parameters such as pH, temperature, or solvent affect the packing of the column material, thereby determining the purification efficiency. The choice of column material also plays an essential role in deciding the operating parameters and can be modified based on the proteins that need to be purified.
Gel Filtration Chromatography
When the...

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Related Experiment Video

Updated: May 27, 2026

Isolation and Characterization Of Chimeric Human Fc-expressing Proteins Using Protein A Membrane Adsorbers And A Streamlined Workflow
10:33

Isolation and Characterization Of Chimeric Human Fc-expressing Proteins Using Protein A Membrane Adsorbers And A Streamlined Workflow

Published on: January 8, 2014

Technology trends in antibody purification.

Pete Gagnon1

  • 1Bioprocessing Technology Institute, 20 Biopolis Way, 06-01 Centros, Singapore 138668. pete gagnon@bti.a-star.edu.sg

Journal of Chromatography. A
|November 11, 2011
PubMed
Summary
This summary is machine-generated.

This review covers antibody purification technologies, including non-chromatography and advanced chromatography methods. It also explores buffer formulation strategies to maintain antibody integrity during purification.

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Activated Cross-linked Agarose for the Rapid Development of Affinity Chromatography Resins - Antibody Capture as a Case Study

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Laboratory Scale Production and Purification of a Therapeutic Antibody
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Laboratory Scale Production and Purification of a Therapeutic Antibody

Published on: January 24, 2017

Related Experiment Videos

Last Updated: May 27, 2026

Isolation and Characterization Of Chimeric Human Fc-expressing Proteins Using Protein A Membrane Adsorbers And A Streamlined Workflow
10:33

Isolation and Characterization Of Chimeric Human Fc-expressing Proteins Using Protein A Membrane Adsorbers And A Streamlined Workflow

Published on: January 8, 2014

Activated Cross-linked Agarose for the Rapid Development of Affinity Chromatography Resins - Antibody Capture as a Case Study
07:53

Activated Cross-linked Agarose for the Rapid Development of Affinity Chromatography Resins - Antibody Capture as a Case Study

Published on: August 16, 2019

Laboratory Scale Production and Purification of a Therapeutic Antibody
09:54

Laboratory Scale Production and Purification of a Therapeutic Antibody

Published on: January 24, 2017

Area of Science:

  • Biotechnology
  • Chemical Engineering
  • Biochemistry

Background:

  • Antibody purification is critical for therapeutic efficacy and safety.
  • Traditional methods face challenges with increasing antibody complexity and production scales.
  • Optimizing purification processes is essential for cost-effective biopharmaceutical manufacturing.

Purpose of the Study:

  • To provide a comprehensive overview of current and emerging antibody purification technologies.
  • To discuss advancements in both chromatographic and non-chromatographic purification techniques.
  • To highlight innovative strategies for maintaining antibody quality during purification.

Main Methods:

  • Review of non-chromatography techniques: precipitation, liquid-liquid extraction, and tangential flow filtration.
  • Detailed analysis of chromatography methods: fluidized/fixed beds, various stationary phase architectures (particles, membranes, monoliths), and specific modes (SEC, IEX, HIC, IMAC, mixed-mode, affinity).
  • Exploration of process buffer formulation for preventing aggregates, dissociating complexes, and restoring native antibody structure.

Main Results:

  • Non-chromatography methods offer alternatives for initial antibody capture and concentration.
  • Innovations in chromatography, including perfusive particles and monoliths, enhance efficiency and resolution.
  • Buffer engineering presents a promising approach to address antibody instability and improve final product quality.

Conclusions:

  • A diverse range of purification technologies are available, with ongoing innovation in chromatography and buffer formulation.
  • Selecting the appropriate purification strategy depends on the specific antibody and desired product profile.
  • Future antibody purification will likely integrate advanced separation techniques with intelligent buffer design for optimal yield and quality.