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Related Concept Videos

Raman Spectroscopy: Overview01:20

Raman Spectroscopy: Overview

The underlying principle of Raman spectroscopy is based on the interaction between light and matter, specifically molecules' inelastic scattering of photons. When a monochromatic beam of light, typically from a laser source, interacts with a sample, most scattered light has the same frequency as the incident light. This is known as Rayleigh scattering.
However, a small fraction of the scattered light exhibits a frequency shift due to the exchange of energy between the incident photons and the...
Raman Spectroscopy Instrumentation: Overview01:26

Raman Spectroscopy Instrumentation: Overview

A conventional Raman spectrophotometer includes a laser source, a sample holding system, a wavelength selector, and a detector.
The monochromatic laser source, typically using visible or near-infrared radiation, generates a highly focused beam of light. This light interacts with the molecules of the sample, scattering some of the light. Liquid and gaseous samples are usually tested in ordinary glass capillaries, while solids can be analyzed as powders packed in capillaries or as potassium...

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Related Experiment Video

Updated: May 27, 2026

Label-free in situ Imaging of Lignification in Plant Cell Walls
07:35

Label-free in situ Imaging of Lignification in Plant Cell Walls

Published on: November 1, 2010

Raman-spectroscopy-based noninvasive microanalysis of native lignin structure.

Pradeep N Perera1, Martin Schmidt, Vincent L Chiang

  • 1Energy Biosciences Institute, University of California, Berkeley, CA 94720, USA. pperera@lbl.gov

Analytical and Bioanalytical Chemistry
|November 11, 2011
PubMed
Summary

A new Raman microspectroscopy method noninvasively analyzes native lignin structure. This technique revealed significant structural differences and quantified changes in lignin composition, such as the syringyl-to-guaiacyl ratio in poplar.

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Measuring Interactions between Fluorescent Probes and Lignin in Plant Sections by sFLIM Based on Native Autofluorescence

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Quantitative 31P NMR Analysis of Lignins and Tannins
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Quantitative 31P NMR Analysis of Lignins and Tannins

Published on: August 2, 2021

Related Experiment Videos

Last Updated: May 27, 2026

Label-free in situ Imaging of Lignification in Plant Cell Walls
07:35

Label-free in situ Imaging of Lignification in Plant Cell Walls

Published on: November 1, 2010

Measuring Interactions between Fluorescent Probes and Lignin in Plant Sections by sFLIM Based on Native Autofluorescence
07:15

Measuring Interactions between Fluorescent Probes and Lignin in Plant Sections by sFLIM Based on Native Autofluorescence

Published on: January 2, 2020

Quantitative 31P NMR Analysis of Lignins and Tannins
05:57

Quantitative 31P NMR Analysis of Lignins and Tannins

Published on: August 2, 2021

Area of Science:

  • Biochemistry
  • Spectroscopy
  • Plant Science

Background:

  • Lignin's complex structure influences plant biomass properties.
  • Accurate analysis of native lignin structure is crucial for biomass utilization.
  • Current methods for lignin structural analysis can be invasive or lack precision.

Purpose of the Study:

  • To introduce a robust, noninvasive Raman microspectroscopic method for native lignin structural analysis.
  • To demonstrate the method's capability in detecting structural differences across plant species.
  • To quantify compositional changes in genetically modified plants and analyze cell-specific lignin distribution.

Main Methods:

  • Utilized Raman microspectroscopy for noninvasive analysis of native lignin.
  • Acquired and analyzed lignin spectra from poplar, Arabidopsis, and Miscanthus.
  • Performed compositional analysis on 4-coumarate-CoA ligase suppressed transgenic poplar.
  • Conducted cell-specific compositional analysis on basal stems of Arabidopsis.

Main Results:

  • Successfully recovered lignin spectra from diverse plant species.
  • Unambiguously detected significant structural differences in native lignin.
  • Quantified a 35% decrease in the syringyl-to-guaiacyl ratio in transgenic poplar.
  • Observed similar distributions of S and G monolignols in different cell types of Arabidopsis.

Conclusions:

  • Raman microspectroscopy provides a powerful tool for noninvasive lignin structural analysis.
  • The method can accurately detect and quantify lignin compositional variations.
  • Findings contribute to understanding lignin biosynthesis and its cell-specific deposition in plants.