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Updated: May 27, 2026
Isolation of Human Mesenchymal Stem Cells and their Cultivation on the Porous Bone Matrix
Published on: February 9, 2015
Co-culturing mesenchymal stem cells from bone marrow and periosteum enhances osteogenesis and neovascularization of
Daoyun Chen1, Xianlong Zhang, Yaohua He
1Department of Orthopaedic Surgery, Shanghai Sixth People's Hospital, Shanghai Jiaotong University, 600 Yishan Road, Shanghai, 200233, P.R., China.
Abstract:
Mesenchymal stem cells (MSCs) isolated from bone marrow and periosteum are often used as cellular sources for bone tissue engineering. This study showed that co-cultured human bone marrow stem cells (hBMSCs) and periosteal-derived stem cells (hPCs) resulted in a synergistic effect on osteogenic differentiation both in vitro and in vivo. Compared to hBMSCs and hPCs, co-culturing MSCs showed abundant mineralization, robust calcium deposition, steadily increasing ALP activity, and upgraded mRNA expression of osteogenic specific genes (COL1A1, BMP-2, osteopontin, osteocalcin) in vitro. Eight weeks after implantation of cellular β-TCP scaffolds in immunodeficient mice, similar synergistic effects were confirmed during in vivo evaluation of total new bone formation, mature bone formation, and neovascularization. Based on these findings, the use of co-cultured hBMSCs and hPCs can be recommended as a promising new approach for bone tissue engineering applications.
