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Related Concept Videos

Sampling Methods: Sample Types01:18

Sampling Methods: Sample Types

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Sampling materials are classified into three main types: solid, liquid, and gas.
Solid samples include a variety of substances, such as sediments from water bodies, soil, metals, and biological tissues. Two standard methods for extracting sediments from water bodies are grab sampling and piston coring. Grab sampling involves using a device to collect a discrete sediment sample from the bottom of a water body with minimal disturbance. Grab samples do not always represent the entire area due to...
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Convenience Sampling Method00:55

Convenience Sampling Method

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Sampling is a technique to select a portion (or subset) of the larger population and study that portion (the sample) to gain information about the population. Data are the result of sampling from a population. The sampling method ensures that samples are drawn without bias and accurately represent the population.
Convenience sampling is a non-random method of sample selection; this method selects individuals that are easily accessible and may result in biased data. For example, a marketing...
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Sampling Methods: Overview01:06

Sampling Methods: Overview

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A sample refers to a smaller subset representative of a larger population. In analytical chemistry, studying or analyzing an entire population is often impractical or impossible. Therefore, samples are used to draw inferences and generalize the whole population. The sampling method selects individuals or items from a population to create a sample. Standard sampling methods include random, judgemental, systematic, stratified, and cluster sampling. 
In analytical chemistry, the choice of...
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Systematic Sampling Method01:17

Systematic Sampling Method

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Sampling is a technique to select a portion (or subset) of the larger population and study that portion (the sample) to gain information about the population. Data are the result of sampling from a population. The sampling method ensures that samples are drawn without bias and accurately represent the population. Because measuring the entire population in a study is not practical, researchers use samples to represent the population of interest.
Systematic sampling is one of the simplest methods...
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Stratified Sampling Method01:16

Stratified Sampling Method

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Sampling is a technique to select a portion (or subset) of the larger population and study that portion (the sample) to gain information about the population. The sampling method ensures that samples are drawn without bias and accurately represent the population. Because measuring the entire population in a study is not practical, researchers use samples to represent the population of interest.
To choose a stratified sample, divide the population into groups called strata and then take a...
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Cluster Sampling Method01:20

Cluster Sampling Method

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Appropriate sampling methods ensure that samples are drawn without bias and accurately represent the population. Because measuring the entire population in a study is not practical, researchers use samples to represent the population of interest.
To choose a cluster sample, divide the population into clusters (groups) and then randomly select some of the clusters. All the members from these clusters are in the cluster sample. For example, if you randomly sample four departments from your...
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Development of Microfluidic Devices to Study the Elongation Capability of Tip-growing Plant Cells in Extremely Small Spaces
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A simultaneous space sampling method for DNA fraction collection using a comb structure in microfluidic devices.

Zheyu Li1, Kai Sun, Misato Sunayama

  • 1Research Institute for Electronic Science, Hokkaido University, Kita-ku, Sapporo, Japan.

Electrophoresis
|November 11, 2011
PubMed
Summary
This summary is machine-generated.

This study presents a novel electrophoretic chip for simultaneous DNA fractionation using space sampling. This breakthrough method efficiently collects pure ssDNA fragments in minutes, improving upon traditional techniques.

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Area of Science:

  • Biotechnology
  • Analytical Chemistry
  • Molecular Biology

Background:

  • Fraction collection is crucial for analyzing complex mixtures in various scientific fields.
  • Existing methods for collecting specific components, like DNA fragments, can be time-consuming and inefficient, especially with closely related molecules.

Purpose of the Study:

  • To develop a novel electrophoretic chip device for simultaneous fractionation of single-stranded DNA (ssDNA) target fragments.
  • To improve the efficiency and purity of fraction collection using a space sampling approach.

Main Methods:

  • Development of a microfluidic electrophoretic chip with ten parallel extraction channels for simultaneous space sampling.
  • Utilizing a signal processing theorem to enable fractionation of ssDNA fragments within short sampling ranges.
  • Collecting fragments of 180, 181, and 182 bases and subsequently analyzing them via PCR amplification and capillary electrophoresis (CE).

Main Results:

  • Simultaneous collection of 180, 181, and 182 base ssDNA fragments was achieved.
  • The 181-base target fragment was isolated within a 70-mm separation length in just 10 minutes.
  • This represents a significant reduction in time compared to previous methods requiring >50 minutes for longer separation channels.

Conclusions:

  • The novel electrophoretic chip effectively combines time and space for efficient fraction collection.
  • This space sampling extraction method offers a breakthrough in chip-based fraction collection, enabling pure collection of even partly overlapping fragments.
  • The developed technology significantly enhances the speed and purity of DNA fragment isolation for research applications.