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Related Experiment Video

Updated: May 27, 2026

Humanized NOD/SCID/IL2rγnull (hu-NSG) Mouse Model for HIV Replication and Latency Studies
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Humanized NOD/SCID/IL2rγnull (hu-NSG) Mouse Model for HIV Replication and Latency Studies

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HIV latency in the humanized BLT mouse.

Matthew D Marsden1, Michael Kovochich, Nuttee Suree

  • 1Department of Medicine, Division of Hematology and Oncology, David Geffen School of Medicine at UCLA, Los Angeles, California, USA.

Journal of Virology
|November 11, 2011
PubMed
Summary

The humanized BLT mouse model effectively harbors latent HIV in CD4(+) T cells, serving as a crucial tool for testing new HIV latency-purging strategies and activators in vivo.

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Last Updated: May 27, 2026

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Chronic, Acute, and Reactivated HIV Infection in Humanized Immunodeficient Mouse Models
09:54

Chronic, Acute, and Reactivated HIV Infection in Humanized Immunodeficient Mouse Models

Published on: December 3, 2019

Area of Science:

  • Immunology
  • Virology
  • Infectious Diseases

Background:

  • Despite antiretroviral therapy, HIV persists in latent reservoirs, primarily in CD4(+) T cells.
  • Eliminating these latent reservoirs is essential for a complete HIV cure.
  • Current in vivo models for testing latency-purging strategies are limited.

Purpose of the Study:

  • To evaluate the humanized bone marrow-liver-thymus (BLT) mouse as an in vivo model for studying HIV latency.
  • To assess the BLT mouse model's utility in testing strategies to eliminate latent HIV reservoirs.

Main Methods:

  • HIV infection of humanized BLT mice.
  • Ex vivo and in vivo analysis of latently infected CD4(+) T cells.
  • Treatment with antiretroviral drugs (zidovudine, indinavir sulfate, didanosine).
  • Induction of HIV reactivation using prostratin and 12-deoxyphorbol-13-phenylacetate.

Main Results:

  • Over 2% of spleen cells in HIV-infected BLT mice were latently infected, replication-competent, and activation-inducible.
  • Prostratin and 12-deoxyphorbol-13-phenylacetate successfully induced HIV ex vivo from these cells.
  • Activation-inducible HIV persisted in BLT mice even after plasma viral loads were suppressed to undetectable levels by antiretroviral drugs.

Conclusions:

  • The HIV-infected BLT mouse model is a valuable system for both ex vivo analysis of primary latently infected cells and in vivo studies of HIV latency.
  • This model can be used to assess the efficacy of latency activators and in vivo purging strategies for eliminating persistent HIV reservoirs.