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Related Experiment Video

Updated: May 27, 2026

Separation and Fractionation of Cell Wall and Cell Membrane Proteins from Mycobacterium tuberculosis for Downstream Protein Analysis

Separation and Fractionation of Cell Wall and Cell Membrane Proteins from Mycobacterium tuberculosis for Downstream Protein Analysis

Published on: September 26, 2025

Structural annotation of Mycobacterium tuberculosis proteome.

Praveen Anand¹, Sandhya Sankaran, Sumanta Mukherjee

¹Department of Biochemistry and Bioinformatics Centre, Indian Institute of Science, Bangalore, India.

|November 11, 2011

Summary

This study computationally annotates Mycobacterium tuberculosis H37Rv proteins, revealing fold distribution and enabling drug discovery. Structural models for 70% of the genome enhance understanding of tuberculosis biology.

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Area of Science:

Structural biology
Genomics
Bioinformatics

Background:

Limited experimentally determined protein structures for Mycobacterium tuberculosis (TB) H37Rv genome.
Need for high-resolution understanding of TB biology through structural information.

Purpose of the Study:

To computationally generate structural annotations for the TB H37Rv genome.
To provide a global perspective on fold distribution and facilitate drug discovery.

Main Methods:

Utilized novel algorithms for binding site detection and genome-scale structural comparison.
Generated and validated structural models for approximately 2877 ORFs (70% of the genome).
Performed functional annotation based on fold assignments, binding site analysis, and motif detection.

Main Results:

Generated structural models covering 70% of the TB H37Rv genome.
Identified 219 predominant folds sufficient for cellular metabolism.
Associated 1728 binding pockets with ligands through structural analysis.

Conclusions:

The structural annotation provides a valuable resource for understanding TB biology and accelerating drug discovery.
The developed annotation pipeline is applicable to other microbial genomes.