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Updated: Aug 7, 2026

Isolation and Characterization of Neutrophils with Anti-Tumor Properties
Published on: June 19, 2015
Release of tumor necrosis factor by human polymorphonuclear leukocytes
J Y Djeu1, D Serbousek, D K Blanchard
1Department of Medical Microbiology and Immunology, University of South Florida, College of Medicine, Tampa 33612.
Human neutrophils (PMN) can produce tumor necrosis factor (TNF) when exposed to Candida albicans. This fungal stimulation triggers TNF release, potentially aiding immune response to control fungal growth.
Area of Science:
- Immunology
- Cell Biology
Background:
- Tumor necrosis factor (TNF) production has been documented in macrophages and natural killer cells.
- Previous research indicated Candida albicans can induce TNF from monocytes and lymphocytes.
Purpose of the Study:
- To investigate the capacity of human polymorphonuclear neutrophils (PMN) to produce TNF.
- To determine the kinetics and regulatory mechanisms of TNF production by PMN in response to Candida albicans.
Main Methods:
- Incubation of human PMN with Candida albicans.
- Measurement of TNF release over time.
- Inhibition studies using actinomycin D, emetine, and cycloheximide.
- Neutralization of TNF using monoclonal antibodies.
Main Results:
- PMN released detectable TNF after 3 hours of incubation with C. albicans, with peak induction between 8-18 hours.
- TNF release was inhibited by RNA and protein synthesis inhibitors (actinomycin D, emetine, cycloheximide).
- Monoclonal antibodies neutralized the TNF produced by PMN.
Conclusions:
- Human PMN are capable of producing TNF in response to fungal stimulation by Candida albicans.
- TNF production by PMN is an active process requiring RNA and protein synthesis.
- This finding suggests a role for PMN-derived TNF in autocrine activation and controlling fungal infections.
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