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Related Concept Videos

Phases of Wound Repair01:28

Phases of Wound Repair

Following injury, the integrity of the injured tissues must be reestablished. For example, in skin tissue, wound repair involves coordination among resident skin cells, blood mononuclear cells, extracellular matrix, growth factors, and cytokines to complete the healing cascade.
Formation of Blood Clot
In case of deep injuries, trauma to blood vessels results in blood loss. In the meantime, phospholipids released from the ruptured endothelial cellular membrane are converted into arachidonic...
Clinical Applications of Epidermal Stem Cells01:19

Clinical Applications of Epidermal Stem Cells

Epidermal stem cells (EpiSCs) are mainly located at the basal layer of the epidermis. These cells repair minor injuries of the skin and replace dead skin cells. However, EpiSCs’ cannot heal severe wounds such as major burns or those from diabetes or hereditary disorders. In such cases, culturing the epidermal stem cells from the patient is possible and has yielded successful treatment options, such as laboratory-grown skin grafts. These grafts are synthesized using a patient’s own EpiSCs...

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Related Experiment Video

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Chessboard-like Burn Wound Healing Model of Mice Based on Digital Heating Device
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Published on: December 27, 2024

Calcium-based nanoparticles accelerate skin wound healing.

Kenichiro Kawai1, Barrett J Larson, Hisako Ishise

  • 1Hagey Laboratory for Pediatric Regenerative Medicine, Stanford University School of Medicine, Stanford, California, United States of America.

Plos One
|November 11, 2011
PubMed
Summary

pH-sensitive calcium nanoparticles accelerate wound healing by promoting fibroblast contraction and proliferation. Intravenous administration of these nanoparticles effectively reduces wound size, offering a novel therapeutic approach for wound repair.

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Published on: September 26, 2019

Area of Science:

  • Biomaterials Science
  • Regenerative Medicine
  • Nanotechnology

Background:

  • Nanoparticles (NPs) possess a hydroxyapatite core capable of binding environmental molecules, influencing calcium levels and homeostasis.
  • Calcium-mediated diseases like atherosclerosis and kidney stones are linked to NPs, suggesting potential roles in other calcium-regulated processes.
  • Cutaneous wound healing is a complex calcium-regulated process that may be modulated by NPs.

Purpose of the Study:

  • To synthesize pH-sensitive calcium-based nanoparticles.
  • To investigate the efficacy of these nanoparticles in enhancing cutaneous wound repair.
  • To elucidate the mechanism by which nanoparticles affect wound healing.

Main Methods:

  • Synthesis of pH-sensitive calcium-based nanoparticles.
  • Induction of dorsal cutaneous wounds in Balb/c mice.
  • Intravenous or topical administration of nanoparticles and quantification of wound closure.
  • Tracking of intravenously administered NPs using FLAG detection.
  • In vitro assessment of NP effects on fibroblast contraction and proliferation.

Main Results:

  • Identification of pH-sensitive calcium-based nanoparticles.
  • Intravenous administration of NPs significantly accelerated wound healing.
  • NPs localized to the wound site and increased fibroblast calcium uptake in vitro.
  • Dose-dependent enhancement of fibroblast-populated collagen lattice contracture and increased fibroblast proliferation.

Conclusions:

  • Intravenously administered calcium-based NPs acutely reduce open wound size through contracture.
  • The contraction effect is hypothesized to be mediated by ionized calcium release from NPs in the acidic wound microenvironment.
  • This study demonstrates a novel therapeutic benefit of calcium-based nanoparticles for wound treatment.