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Related Experiment Video

Updated: May 27, 2026

Real-time Live Imaging of T-cell Signaling Complex Formation
10:31

Real-time Live Imaging of T-cell Signaling Complex Formation

Published on: June 23, 2013

Dissecting T-cell activation with high-resolution live-cell microscopy.

Joseph J Illingworth1, P Anton van der Merwe

  • 1Sir William Dunn School of Pathology, University of Oxford, Oxford, UK.

Immunology
|November 15, 2011
PubMed
Summary
This summary is machine-generated.

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Live-cell microscopy reveals T-cell activation dynamics differ in vitro versus in vivo. In situ observation and super-resolution microscopy are crucial for understanding cellular microcluster migration and T-cell responses.

Area of Science:

  • Immunology
  • Cell Biology
  • Microscopy

Background:

  • In vitro studies of T-cell activation machinery components may not accurately reflect in vivo cellular behavior.
  • Understanding T-cell microcluster migration requires in situ observation at the cellular scale.

Purpose of the Study:

  • To investigate the in situ dynamics of T-cell activation using advanced microscopy techniques.
  • To explore the formation and behavior of protein nanodomains during T-cell activation.

Main Methods:

  • Live-cell microscopy
  • Super-resolution microscopy techniques
  • Multifaceted approach to mitigate microscopy-induced artifacts

Main Results:

  • Observed differences between in vitro isolated component behavior and in situ cellular dynamics.

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Visualizing Surface T-Cell Receptor Dynamics Four-Dimensionally Using Lattice Light-Sheet Microscopy

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Spatial and Temporal Control of T Cell Activation Using a Photoactivatable Agonist
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Related Experiment Videos

Last Updated: May 27, 2026

Real-time Live Imaging of T-cell Signaling Complex Formation
10:31

Real-time Live Imaging of T-cell Signaling Complex Formation

Published on: June 23, 2013

Visualizing Surface T-Cell Receptor Dynamics Four-Dimensionally Using Lattice Light-Sheet Microscopy
09:24

Visualizing Surface T-Cell Receptor Dynamics Four-Dimensionally Using Lattice Light-Sheet Microscopy

Published on: January 30, 2020

Spatial and Temporal Control of T Cell Activation Using a Photoactivatable Agonist
07:48

Spatial and Temporal Control of T Cell Activation Using a Photoactivatable Agonist

Published on: April 25, 2018

  • Identified protein islands and nanodomains in both quiescent and active T cells using super-resolution microscopy.
  • Highlighted the importance of in situ observation for accurate understanding of T-cell activation.

    • Conclusions:

    • In situ observation is essential for understanding T-cell activation dynamics.
    • Super-resolution microscopy enables visualization of nanoscale protein organization.
    • Future work should focus on integrating findings into a comprehensive model of T-cell activation and antigen recognition.