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Accelerated high-resolution differential ion mobility separations using hydrogen.

Alexandre A Shvartsburg1, Richard D Smith

  • 1Biological Sciences Division, Pacific Northwest National Laboratory, P.O. Box 999, Richland, Washington 99352, USA. alexandre.shvartsburg@pnl.gov

Analytical Chemistry
|November 15, 2011
PubMed
Summary
This summary is machine-generated.

New research utilizes hydrogen gas in differential ion mobility spectrometry (FAIMS) to significantly boost separation power. This advancement offers faster analysis and finer resolution for complex mixtures, overcoming limitations of previous methods.

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Area of Science:

  • Analytical Chemistry
  • Physical Chemistry

Background:

  • Differential ion mobility spectrometry (FAIMS) resolution has been enhanced using helium or vapor carrier gases.
  • Existing high-resolution FAIMS methods often sacrifice analytical speed, limiting compatibility with online separations.

Purpose of the Study:

  • To investigate the use of hydrogen (H2) in N2 mixtures as a carrier gas for FAIMS.
  • To achieve higher resolving power and faster analysis speeds than previously possible.

Main Methods:

  • FAIMS experiments were conducted using carrier gas mixtures of nitrogen (N2) and hydrogen (H2), with H2 fractions up to 90%.
  • Ion mobilities and resolving power were measured and compared across different H2 concentrations.

Main Results:

  • Hydrogen-rich carrier gases significantly increased ion mobilities and FAIMS resolving power, exceeding previous capabilities.
  • Resolving power gains were most pronounced at H2 fractions above 50%.
  • Analysis speed could be accelerated up to 4 times by trading resolution, particularly for mobile ions like multiply charged peptides.

Conclusions:

  • FAIMS with hydrogen-nitrogen mixtures offers a pathway to unprecedented resolving power and tunable analysis speeds.
  • This approach avoids electrical breakdown issues associated with helium-rich mixtures.
  • Future FAIMS designs optimized for hydrogen could universally enhance resolution for diverse analytes.