Related Experiment Video

Updated: May 27, 2026

The Use of Mouse Mammary Tumor Cells in an In Vitro Invasion Assay as a Measure of Oncogenic Cell Behavior
08:12

The Use of Mouse Mammary Tumor Cells in an In Vitro Invasion Assay as a Measure of Oncogenic Cell Behavior

Published on: June 12, 2019

Metastasis-associated protein 3 (MTA3) regulates G2/M progression in proliferating mouse granulosa cells

Jakub Kwintkiewicz1, Elizabeth Padilla-Banks, Wendy N Jefferson

  • 1Reproductive Medicine Group, Laboratory of Reproductive and Developmental Toxicology, National Institute of Environmental Health Sciences, National Institutes of Health, Research Triangle Park, North Carolina, USA.

Biology of Reproduction
|November 15, 2011
PubMed

Insights

Metastasis-associated protein 3 (MTA3) is crucial for granulosa cell proliferation in the ovary. MTA3 regulates cell cycle progression, impacting ovarian follicle development and function.

Area of Science:

  • Molecular Biology
  • Cell Biology
  • Reproductive Biology

Background:

  • Metastasis-associated protein 3 (MTA3) is part of the Mi-2/nucleosome remodeling and deacetylase (NuRD) complex.
  • The specific role of MTA3 within the NuRD complex and its function in ovarian cells remain largely unknown.

Purpose of the Study:

  • To investigate the function of MTA3 within the NuRD complex in ovarian granulosa cells.
  • To determine if MTA3 is required for granulosa cell proliferation and cell cycle progression.

Main Methods:

  • Immunohistochemistry to assess MTA3 expression in ovarian follicles.
  • RNA interference to deplete MTA3 in primary mouse granulosa cells.
  • Cell proliferation assays, cell cycle analysis (flow cytometry), and Western blotting for cell cycle regulators.

Main Results:

  • MTA3 is highly expressed in granulosa cell nuclei throughout ovarian follicle development.
  • Depletion of MTA3 in granulosa cells significantly slowed proliferation and decreased cyclin B1/B2 expression.
  • MTA3-deficient cells showed altered cell cycle distribution with more cells in G2/M and fewer in S phase, and delayed entry into M phase.

Conclusions:

  • MTA3 interacts with NuRD and cohesin complex proteins in the ovary.
  • MTA3 plays a critical role in regulating granulosa cell proliferation and G2/M phase progression.
  • These findings elucidate a novel function for MTA3 in ovarian cell cycle regulation.

More Related Videos

Tropomodulin 3 Overexpression as a Marker for Platinum Resistance and Immune Infiltration in Ovarian Cancer09:40

Tropomodulin 3 Overexpression as a Marker for Platinum Resistance and Immune Infiltration in Ovarian Cancer

Published on: August 2, 2024

An Orthotopic Murine Model of Human Prostate Cancer Metastasis06:48

An Orthotopic Murine Model of Human Prostate Cancer Metastasis

Published on: September 18, 2013

Related Concept Videos

mTOR Signaling and Cancer Progression03:03

mTOR Signaling and Cancer Progression

The mammalian target of rapamycin or mTOR protein was discovered in 1994 due to its direct interaction with rapamycin. The protein gets its name from a yeast homolog called TOR. The mTOR protein complex in mammalian cells plays a major role in balancing anabolic processes such as the synthesis of proteins, lipids, and nucleotides and catabolic processes, such as autophagy in response to environmental cues, such as availability of nutrients and growth factors.
The mTOR pathway or the...
mTOR Signaling and Cancer Progression03:03

mTOR Signaling and Cancer Progression

The mammalian target of rapamycin or mTOR protein was discovered in 1994 due to its direct interaction with rapamycin. The protein gets its name from a yeast homolog called TOR. The mTOR protein complex in mammalian cells plays a major role in balancing anabolic processes such as the synthesis of proteins, lipids, and nucleotides and catabolic processes, such as autophagy in response to environmental cues, such as availability of nutrients and growth factors.
The mTOR pathway or the...
Abnormal Proliferation02:23

Abnormal Proliferation

Under normal conditions, most adult cells remain in a non-proliferative state unless stimulated by internal or external factors to replace lost cells. Abnormal cell proliferation is a condition in which the cell's growth exceeds and is uncoordinated with normal cells. In such situations, cell division persists in the same excessive manner even after cessation of the stimuli, leading to persistent tumors. The tumor arises from the damaged cells that replicate to pass the damage to the daughter...
PI3K/mTOR/AKT Signaling Pathway01:22

PI3K/mTOR/AKT Signaling Pathway

The mammalian target of rapamycin  (mTOR) is a serine/threonine kinase that regulates growth, proliferation, and cell survival in response to hormones, growth factors, or nutrient availability. This kinase exists in two structurally and functionally distinct forms: mTOR complex 1  (mTORC1) and mTOR complex 2  (mTORC2). The first form (mTORC1) is composed of a rapamycin-sensitive Raptor and proline-rich Akt substrate, PRAS40. In contrast,  mTORC2 consists of a rapamycin-insensitive companion...
M-Cdk Drives Transition Into Mitosis02:15

M-Cdk Drives Transition Into Mitosis

Checkpoints throughout the cell cycle serve as safeguards and gatekeepers, allowing the cell cycle to progress in favorable conditions and slow or halt it in problematic ones. This regulation is known as the cell cycle control system.
Cyclin-dependent kinases, or Cdks, work in concert with cyclins to control cell cycle transitions. M-Cdk, a complex of Cdk1 bound to M cyclin, is a well-known example of this coordinated control that drives the transition from the G2 to the M phase.
M cyclin...