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Updated: May 27, 2026

Combining Analysis of DNA in a Crude Virion Extraction with the Analysis of RNA from Infected Leaves to Discover New Virus Genomes
Published on: July 27, 2018
New Iranian and Australian peach latent mosaic viroid variants and evidence for rapid sequence evolution
Arezou Yazarlou1, Behrooz Jafarpour, Saeed Tarighi
1Department of Plant Pathology, Ferdowsi University of Mashhad, Mashhad, Iran. arezou.yazarlou@adelaide.edu.au
Abstract:
Peach latent mosaic viroid isolates from peach and plum in Iran have been compared with an Australian isolate from nectarine. Thirteen sequence variants 336-338 nt in size were obtained. All variants clustered phylogenetically with variants reported from several hosts and countries. A total nucleic acid extract, a slightly longer than full-length RT-PCR amplicon, and a recombinant plasmid clone from the Australian isolate were all infectious to, and symptomatic in, mechanically inoculated peach seedlings. The infectious clone generated two progeny viroid molecules, which each showed 10 different mutations compared with the parent clone inoculated 30 days previously.
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