Related Experiment Video
Updated: Jan 22, 2026

Non-Aqueous Isolation and Enrichment of Glandular Capitate Stalked and Sessile Trichomes from Cannabis sativa
Published on: May 12, 2023
Complete sequence of a cryptic virus from hemp (Cannabis sativa)
Angelika Ziegler1, Jaroslav Matoušek, Gerhard Steger
1Institute for Biosafety of Genetically Modified Plants, Julius Kühn-Institute, Erwin-Baur-Str. 27, 06484 Quedlinburg, Germany. angelika.ziegler@jki.bund.de
Abstract:
Hemp (Cannabis sativa) was found to be a useful propagation host for hop latent virus, a carlavirus. However, when virus preparations were analysed by electron microscopy, along with the expected filamentous particles, spherical particles with a diameter of around 34 nm were found. RNA from virus preparations was purified, and cDNA was prepared and cloned. Sequence information was used to search databases, and the greatest similarity was found with Primula malacoides virus 1, a putative new member of the genus Partitivirus. The full sequences of RNA 1 and RNA 2 of this new hemp cryptic virus were obtained.
Related Concept Videos
What are Viruses?
Cis-regulatory Sequences
Cis-regulatory Sequences
Sanger Sequencing
Next-generation Sequencing
Next-Generation Sequencing Methods
Although all next-generation methods use different technologies, they all share a set of standard features....
Maxam-Gilbert Sequencing
Challenges of the Maxam-Gilbert Method
The...

