[Identification and expression analysis of a novel splice variant of human tumor necrosis factor receptor associated...

Identification of a novel splice variant of tumor necrosis factor receptor associated factor 2 and its expression analysis.

By PCR analysis, we identified the existence of a new splice variant of TRAF2 using human brain cDNA library as template. RNA isolated from various cell lines and tissues was subjected to RT-PCR and differential expression analysis of TRAF2 splice variants was carried out.

An amplification of about 1 500 bp was found using P1 and P2 as the primers. we performed PCR using exon 6 flanking primers P3 and P4, yielding a full-length transcript of 268 bp (including exon 6) and a novel splice variant of 193 bp lacking the 75 bp of exon 6. The full-length transcript of TRAF2 was found to be dominant in T47D, glioma of grade II and grade III. However the novel splice lacking exon 6 was dominant in Hep3B, GC-1, MCF7, fetus brain and glioma of grade I, the two splices of TRAF2 were similar in PANCI, Hek293 and SW480.We couldn't find any expression of TRAF2 in HepG2, HBL100, A549 and HeLa .

TRAF2 has a novel splice lacking 6 exon in human beside the full-length splice. The two splices are differential expression in tissues and cell lines.