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Updated: May 27, 2026
An Automated Culture System for Use in Preclinical Testing of Host-Directed Therapies for Tuberculosis
Published on: August 16, 2021
[Study on macrophages infected with Mycobacterium tuberculosis H37Ra in vitro]
Lai-cheng Liu1, Shu-ling Wang, Xian-yu Lu
1Department of Medical Laboratory, Children's Hospital of Chongqing Medical University, Chongqing, China. laichengliu@126.com
Aim:
To study the production of nitric oxide and secretion of cytokines after infection of macrophages with Mycobacterium tuberculosis H37Ra.
Methods:
24 hours after infection of RAW264.7 cells with Mycobacterium tuberculosis H37Ra, the production of NO and H(2);O(2); as well as the secretion levels of IL-12 and TNF-α in the supernatants of culture were determined by Griess method, chemical method and ELISA assay respectively. The expression of IL-12 and TNF-α mRNA in macrophages was detected by reverse transcription polymerase chain reaction (RT- PCR).
Results:
Macrophages infected with Mycobacterium tuberculosis H37Ra produced effectively more NO, H(2);O(2);, and enhanced the release of IL-12, TNF-α and the expression of IL-12, TNF-α mRNA (P<0.05).
Conclusion:
Mycobacterium tuberculosis H37Ra can induce the production of more nitric oxide and cytokines which play important roles in the host immune response.
