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Preparation of Naringenin Solution for In Vivo Application
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Published on: August 10, 2021

Updates on naringinase: structural and biotechnological aspects.

Munish Puri1

  • 1Centre for Biotechnology, Chemistry and System Biology (Biodeakin), Institute of Technology Research and Innovation (ITRI), Deakin University, Warrnambool, Victoria, Australia. munish.puri@deakin.edu.au

Applied Microbiology and Biotechnology
|November 15, 2011
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Summary

Bacterial naringinases, enzymes that break down specific sugars, offer promising industrial applications. Research focuses on optimizing their production and engineering for enhanced biotechnological uses.

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Area of Science:

  • Biochemistry
  • Enzymology
  • Microbiology

Background:

  • Naringinases are enzymes with significant hydrolytic activities, including rhamnose production and citrus juice debittering.
  • While fungal naringinases are well-studied and industrially utilized, bacterial sources are less explored.
  • Bacterial rhamnosidases, belonging to glycoside hydrolase family 78, are exotype enzymes crucial for hydrolyzing specific glycosidic bonds.

Purpose of the Study:

  • To discuss the production of bacterial naringinase.
  • To explore the potential biotechnological applications of bacterial naringinases.
  • To highlight the structural characteristics and catalytic mechanisms of bacterial rhamnosidases.

Main Methods:

  • Review of literature on bacterial naringinase production.
  • Analysis of structural data for glycoside hydrolase family 78.
  • Discussion of optimization strategies for fermentation and enzyme engineering.

Main Results:

  • Bacterial naringinases, structurally characterized by Asp567 and Glu841, offer alternative production routes compared to fungi.
  • These enzymes effectively hydrolyze terminal non-reducing α-L-rhamnosyl groups from various substrates.
  • Potential applications in food processing and biotechnology are identified.

Conclusions:

  • Bacterial naringinases present a viable alternative to fungal counterparts for industrial applications.
  • Further optimization of production and enzyme engineering can enhance their utility.
  • Advancements in understanding bacterial rhamnosidases will drive their biotechnological development.