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Embryonic Stem Cells

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Related Experiment Video

Updated: May 27, 2026

Prediction and Validation of Gene Regulatory Elements Activated During Retinoic Acid Induced Embryonic Stem Cell Differentiation

Prediction and Validation of Gene Regulatory Elements Activated During Retinoic Acid Induced Embryonic Stem Cell Differentiation

Published on: June 21, 2016

Embryonic stem cell-based mapping of developmental transcriptional programs.

Esteban O Mazzoni¹, Shaun Mahony, Michelina Iacovino

¹Departments of Pathology, Neurology and Neuroscience, Center for Motor Neuron Biology and Disease and Columbia Stem Cell Initiative, Columbia University Medical Center, New York, New York, USA.

|November 15, 2011

Summary

Researchers developed a new method to map transcription factor binding sites using stem cell differentiation and inducible protein expression. This technique overcomes challenges in antibody availability and cell numbers for ChIP-seq studies.

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Area of Science:

Developmental biology
Genomics
Molecular biology

Background:

Chromatin immunoprecipitation and deep sequencing (ChIP-seq) is crucial for studying transcription factor binding.
Current ChIP-seq methods face limitations due to antibody availability and cell number requirements.

Purpose of the Study:

To develop a versatile method for genome-wide analysis of transcription-factor binding sites.
To overcome existing obstacles in ChIP-seq studies of developmentally regulated factors.

Main Methods:

Combining directed differentiation of embryonic stem cells with inducible expression of tagged proteins.
Utilizing the developed method for mapping DNA-binding sites.

Main Results:

Successfully mapped DNA-binding sites of transcription factors involved in motor neuron specification.
Demonstrated the utility of the combined approach.

Conclusions:

The described method provides a versatile approach for genome-wide transcription factor analysis.
This technique enhances ChIP-seq applicability, particularly for developmentally regulated factors and limited cell samples.