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Related Concept Videos

Cleavage and Blastulation01:33

Cleavage and Blastulation

After a large-single-celled zygote is produced via fertilization, the process of cleavage occurs while zygotes travel through the uterine tube. Cleavage is a mitotic cell division that does not result in growth. With each round of successive cell division, daughter cells get increasingly smaller.
Gastrulation01:56

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Gastrulation establishes the three primary tissues of an embryo: the ectoderm, mesoderm, and endoderm. This developmental process relies on a series of intricate cellular movements, which in humans transforms a flat, “bilaminar disc” composed of two cell sheets into a three-tiered structure. In the resulting embryo, the endoderm serves as the bottom layer, and stacked directly above it is the intermediate mesoderm, and then the uppermost ectoderm. Respectively, these tissue strata will form...

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Protocol for Human Blastoids Modeling Blastocyst Development and Implantation
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Human embryonic development after blastomere removal: a time-lapse analysis.

Kirstine Kirkegaard1, Johnny Juhl Hindkjaer, Hans Jakob Ingerslev

  • 1Centre for Preimplantation Genetic Diagnosis, The Fertility Clinic, Aarhus University Hospital, Skejby, Brendstrupgaardsvej 100, 8200 Aarhus N, Denmark. kirstine.kirkegaard@ki.au.dk

Human Reproduction (Oxford, England)
|November 15, 2011
PubMed
Summary

Blastomere biopsy for preimplantation genetic diagnosis (PGD) delays human embryonic development and alters hatching mechanisms. This study used time-lapse imaging to compare biopsied and non-biopsied embryos, revealing significant developmental delays and changes in hatching behavior.

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Area of Science:

  • Reproductive biology
  • Embryology
  • Genetics

Background:

  • Blastomere biopsy is crucial for preimplantation genetic diagnosis (PGD).
  • Its impact on early human embryonic development remains largely uncharacterized.
  • Mouse studies suggest potential effects on hatching.

Purpose of the Study:

  • To evaluate the effect of blastomere biopsy on early human embryonic development.
  • To utilize time-lapse analysis for detailed observation of embryonic progression.

Main Methods:

  • Compared 56 biopsied human embryos (PGD group) with 53 non-biopsied controls (IVF/ICSI).
  • Cultured all embryos for 5 days post-fertilization in a time-lapse incubator.
  • Registered key embryonic event time-points for comparative analysis.

Main Results:

  • Biopsied embryos showed a prolonged cell-stage and delayed subsequent developmental milestones (compaction, morula, blastocyst formation).
  • Hatching initiation time was similar, but biopsied embryos exhibited smaller diameter and thicker zona pellucida.
  • Hatching mechanism differed: controls expanded zona pellucida, while PGD embryos utilized the biopsy site opening.

Conclusions:

  • Blastomere biopsy prolongs the biopsied cell-stage, potentially due to delayed compaction.
  • The hatching mechanism is altered following blastomere biopsy.
  • Time-lapse imaging provides critical insights into biopsy-induced developmental changes.