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Related Concept Videos

DNA Microarrays02:34

DNA Microarrays

Microarrays are high-throughput and relatively inexpensive assays that can be automated to analyze large quantities of data at a time. They are used in genome-wide studies to compare gene or protein expression under two varied conditions, such as healthy and diseased states. Microarrays consist of glass or silica slides on which probe molecules are covalently attached through surface functionalization. Most commonly, the slides are prepared through the chemisorption of silanes to silica...
RNA-seq03:21

RNA-seq

RNA sequencing, or RNA-Seq, is a high-throughput sequencing technology used to study the transcriptome of a cell. Transcriptomics helps to interpret the functional elements of a genome and identify the molecular constituents of an organism. Additionally, it also helps in understanding the development of an organism and the occurrence of diseases. 
Before the discovery of RNA-seq, microarray-based methods and Sanger sequencing were used for transcriptome analysis. However, while microarray-based...
Ribosome Profiling02:24

Ribosome Profiling

Ribosome profiling or ribo-sequencing is a deep sequencing technique that produces a snapshot of active translation in a cell. It selectively sequences the mRNAs protected by ribosomes to get an insight into a cell’s translation landscape at any given point in time.
Applications of ribosome profiling
Ribosome profiling has many applications, including in vivo monitoring of translation inside a particular organ or tissue type and quantifying new protein synthesis levels.
The technique helps...

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Related Experiment Video

Updated: May 27, 2026

DNA Microarrays: Sample Quality Control, Array Hybridization and Scanning
09:27

DNA Microarrays: Sample Quality Control, Array Hybridization and Scanning

Published on: March 15, 2011

Genomic profiling: cDNA arrays and oligoarrays.

Francesco Gorreta1, Walter Carbone, Dagania Barzaghi

  • 1Genetics and Biomarkers-Exploratory Medicine, Merck Serono Ivrea, Colleretto Giacosa-TO, Italy. francesco.gorreta@merckserono.net

Methods in Molecular Biology (Clifton, N.J.)
|November 15, 2011
PubMed
Summary

Microarray technology enables simultaneous analysis of many nucleic acid transcripts for disease diagnosis and tumor profiling. Standards ensure quality control across diverse platforms and experimental variability.

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Area of Science:

  • Molecular Biology
  • Genomics
  • Biotechnology

Background:

  • Microarray technology offers simultaneous analysis of numerous nucleic acid transcripts.
  • This massively parallel analysis is crucial for disease diagnosis and molecular tumor profiling.
  • Applications include gene expression, SNP detection, CGH, DNA methylation, and microRNA evaluation.

Purpose of the Study:

  • To review microarray construction, methodologies, and applications.
  • To highlight the importance of microarray standards for quality control.
  • To provide information relevant to molecular profiling using microarrays.

Main Methods:

  • Review of commercial microarray platforms and construction methods.
  • Description of typical experimental workflow: sample preparation, array construction, hybridization, detection, and analysis.
  • Discussion of factors influencing experimental variability.

Main Results:

  • Microarrays facilitate diverse genomic analyses, including gene expression and methylation.
  • Standardization is essential due to platform variety and experimental variability.
  • The chapter provides a comprehensive overview of microarray techniques.

Conclusions:

  • Microarray technology is indispensable for advanced molecular profiling and diagnostics.
  • Adherence to standards is critical for reliable and reproducible microarray results.
  • The reviewed methodologies and applications underscore the power of microarrays in biological research.