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Related Experiment Videos

ras gene mutations in human prostate cancer.

B S Carter1, J I Epstein, W B Isaacs

  • 1Brady Urological Research Institute, Johns Hopkins University School of Medicine and Hospital, Baltimore, Maryland 21205.

Cancer Research
|November 1, 1990
PubMed
Summary

Ras gene mutations, though infrequent in prostate cancer, can activate oncogenes. These mutations may play a role in the progression of prostate tumors, including rare ductal adenocarcinomas.

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Area of Science:

  • Oncology
  • Molecular Biology
  • Genetics

Background:

  • Point mutations in ras genes (Ha-, Ki-, and N-ras) can activate them as oncogenes.
  • Ras gene mutations are implicated in the pathogenesis of various human solid tumors.

Purpose of the Study:

  • To investigate the association between ras gene mutations and prostate cancer.
  • To determine if ras gene mutations are present in prostate tumors and cell lines.

Main Methods:

  • Oligodeoxynucleotide hybridization assay used to detect ras gene mutations.
  • Genomic DNA from 24 primary prostate tumors and 5 cell lines amplified via polymerase chain reaction.
  • Analysis focused on codons 12, 13, and 61 of Ha-, Ki-, and N-ras genes.

Main Results:

  • Two ras gene mutations were identified in the examined prostate samples.
  • An Ha-ras codon 61 mutation (A-G transition) was found in a primary ductal adenocarcinoma.
  • An Ha-ras codon 12 mutation (G-T transversion) was detected in a prostate cancer cell line (TSU-PR1) from a metastasis.

Conclusions:

  • Ras gene mutations are rare in prostate cancer but may contribute to disease progression.
  • These mutations might be involved in the development of the ductal variant of prostatic adenocarcinoma.

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