Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Overview of Microscopy Techniques01:22

Overview of Microscopy Techniques

The early pioneers of microscopy opened a window into the invisible world of microorganisms. In 1830, Joseph Jackson Lister created an essentially modern light microscope. The 20th century saw the development of microscopes that leveraged nonvisible light, such as fluorescence microscopy that uses an ultraviolet light source and electron microscopy that uses short-wavelength electron beams. These advances significantly improved magnification, image resolution, and contrast. By comparison, the...
Phase Contrast and Differential Interference Contrast Microscopy01:26

Phase Contrast and Differential Interference Contrast Microscopy

Phase-Contrast Microscopes
In-phase-contrast microscopes, interference between light directly passing through a cell and light refracted by cellular components is used to create high-contrast, high-resolution images without staining. It is the oldest and simplest type of microscope that creates an image by altering the wavelengths of light rays passing through the specimen. Altered wavelength paths are created using an annular stop in the condenser. The annular stop produces a hollow cone of...
Atomic Force Microscopy01:08

Atomic Force Microscopy

Atomic force microscopy (AFM) is a type of scanning probe microscopy that can analyze topographic details of various specimens like ceramics, glass, polymers, and biological samples. AFM offers over 1000 times more resolution than the optical imaging system. Images generated from AFM are three-dimensional surface profiles, offering an advantage over the flat, two-dimensional images from other imaging techniques.
The AFM Probe
The probe is regarded as the heart of any AFM setup and comprises the...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Pancreatic Cancer Immunotherapy: A Team-Based Approach.

Cancer treatment and research·2025
Same author

Risk of Open Conversion During Robotic Gastrectomy for Gastric Cancer: Optimizing Patient Selection.

Journal of surgical oncology·2025
Same author

Robotic pancreatectomy for pancreatic adenocarcinoma: evolving trends in patient selection and practice patterns across a decade.

HPB : the official journal of the International Hepato Pancreato Biliary Association·2025
Same author

Kidney Stone Dissolution By Tetherless, Enzyme-Loaded, Soft Magnetic Miniature Robots.

Advanced healthcare materials·2025
Same author

Quantum Elastica.

Entropy (Basel, Switzerland)·2025
Same author

Randomized Controlled Trial-Perioperative Telemonitoring of Patient-Generated Health Data in Gastrointestinal Oncologic (GI) Surgery: Assessing Outcomes.

Annals of surgery·2025

Related Experiment Video

Updated: May 27, 2026

Time Multiplexing Super Resolving Technique for Imaging from a Moving Platform
06:25

Time Multiplexing Super Resolving Technique for Imaging from a Moving Platform

Published on: February 12, 2014

Noniterative exact solution to the phase problem in optical imaging implemented with scanning probe microscopy.

Danielle R Honigstein1, Jacques Weinroth, Michael Werman

  • 1Department of Applied Physics, Hebrew University of Jerusalem, Israel.

ACS Nano
|November 16, 2011
PubMed
Summary

Researchers developed a new optical phase retrieval method inspired by crystallography. This technique uses a scanning probe microscope to restore lost phase information, enabling advanced 3D optical imaging with super-resolution potential.

More Related Videos

Shaping the Amplitude and Phase of Laser Beams by Using a Phase-only Spatial Light Modulator
08:39

Shaping the Amplitude and Phase of Laser Beams by Using a Phase-only Spatial Light Modulator

Published on: January 28, 2019

Quantitative Optical Microscopy: Measurement of Cellular Biophysical Features with a Standard Optical Microscope
14:09

Quantitative Optical Microscopy: Measurement of Cellular Biophysical Features with a Standard Optical Microscope

Published on: April 7, 2014

Related Experiment Videos

Last Updated: May 27, 2026

Time Multiplexing Super Resolving Technique for Imaging from a Moving Platform
06:25

Time Multiplexing Super Resolving Technique for Imaging from a Moving Platform

Published on: February 12, 2014

Shaping the Amplitude and Phase of Laser Beams by Using a Phase-only Spatial Light Modulator
08:39

Shaping the Amplitude and Phase of Laser Beams by Using a Phase-only Spatial Light Modulator

Published on: January 28, 2019

Quantitative Optical Microscopy: Measurement of Cellular Biophysical Features with a Standard Optical Microscope
14:09

Quantitative Optical Microscopy: Measurement of Cellular Biophysical Features with a Standard Optical Microscope

Published on: April 7, 2014

Area of Science:

  • Optical Physics
  • Microscopy
  • Crystallography

Background:

  • Optical imaging typically loses phase information, limiting object characterization.
  • The "heavy atom" method in crystallography enabled phase retrieval for large molecules.
  • Advancements in scanning probe microscopy offer new possibilities for optical phase retrieval.

Purpose of the Study:

  • To develop a novel optical phase retrieval method.
  • To adapt crystallographic "heavy atom" concepts for optical imaging.
  • To achieve robust 3D optical imaging with super-resolution capabilities.

Main Methods:

  • Utilized near-field scanning optical microscopy (NSOM) integrated with atomic force microscopy (AFM).
  • Employed a nanometrically translatable point source of light as an analog to "heavy atoms".
  • Acquired Fourier intensities analogous to X-ray diffraction patterns for phase retrieval.

Main Results:

  • Successfully implemented "heavy atom" restoration of phase in optical phase retrieval.
  • Achieved robust phase retrieval independent of external parameters.
  • Demonstrated the potential for 3D optical imaging and super-resolution.

Conclusions:

  • The "heavy atom" restoration of phase with super-resolution (HARPS) method enables advanced optical imaging.
  • Integration of NSOM/AFM with optical imaging provides a powerful tool for phase retrieval.
  • This methodology holds potential for integration with other imaging modalities like electron or ion microscopy.