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Related Experiment Video

Updated: May 27, 2026

Targeted DNA Methylation Analysis by Next-generation Sequencing
08:38

Targeted DNA Methylation Analysis by Next-generation Sequencing

Published on: February 24, 2015

Whole Genome Methylation Scanning Based on phi29 Polymerase Amplification.

R Brooks1, R J Rose, M B Sheahan

  • 1Australian Research Council Centre of Excellence for Integrative Legume Research, School of Environmental and Life Sciences, The University of Newcastle, Callaghan, New South Wales, Australia.

Biochemistry. Biokhimiia
|November 16, 2011
PubMed
Summary

This study introduces a novel method to differentiate methylated and unmethylated DNA using a single restriction enzyme. This technique aids in understanding epigenetic regulation of gene expression during development.

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Area of Science:

  • Epigenetics and Molecular Biology
  • Genomics and Bioinformatics

Background:

  • DNA methylation is a key epigenetic mechanism regulating gene expression.
  • Understanding methylation patterns is crucial for studying development and disease.

Purpose of the Study:

  • To develop a novel method for isolating methylated and unmethylated DNA fractions from a single sample.
  • To enable precise analysis of epigenetic modifications and their impact on gene expression.

Main Methods:

  • Utilizes a single methylation-sensitive restriction endonuclease for differential DNA digestion.
  • Employs concatemerization with stuffer-adaptor DNA to isolate unmethylated fragments.
  • Employs phi29 polymerase amplification and re-digestion to isolate methylated fragments.

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Optimized Analysis of DNA Methylation and Gene Expression from Small, Anatomically-defined Areas of the Brain
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Optimized Analysis of DNA Methylation and Gene Expression from Small, Anatomically-defined Areas of the Brain

Published on: July 12, 2012

Genome-Wide Analysis of DNA Methylation in Gastrointestinal Cancer
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Genome-Wide Analysis of DNA Methylation in Gastrointestinal Cancer

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Related Experiment Videos

Last Updated: May 27, 2026

Targeted DNA Methylation Analysis by Next-generation Sequencing
08:38

Targeted DNA Methylation Analysis by Next-generation Sequencing

Published on: February 24, 2015

Optimized Analysis of DNA Methylation and Gene Expression from Small, Anatomically-defined Areas of the Brain
13:11

Optimized Analysis of DNA Methylation and Gene Expression from Small, Anatomically-defined Areas of the Brain

Published on: July 12, 2012

Genome-Wide Analysis of DNA Methylation in Gastrointestinal Cancer
07:50

Genome-Wide Analysis of DNA Methylation in Gastrointestinal Cancer

Published on: September 18, 2020

Main Results:

  • Successfully generated distinct pools of methylated and unmethylated DNA fragments.
  • Demonstrated the feasibility of the proposed method through a proof-of-concept experiment.
  • The method allows for downstream analysis via end-sequencing or genomic array hybridization.

Conclusions:

  • The developed method provides a robust approach for dissecting DNA methylation patterns.
  • This technique facilitates a deeper understanding of epigenetics in biological processes.
  • Offers a valuable tool for epigenetic research and biomarker discovery.