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Updated: May 27, 2026

Collection and Extraction of Occupational Air Samples for Analysis of Fungal DNA
12:02

Collection and Extraction of Occupational Air Samples for Analysis of Fungal DNA

Published on: May 2, 2018

Aspergillus hazardous problem in ceramic workers.

Amal Saad-Hussein1, Nadia Y S Morcos, Sanaa A Rizk

  • 1Department of Environmental and Occupational Medicine, National Research Centre, Egypt. amel_h@hotmail.com

Toxicology and Industrial Health
|November 16, 2011
PubMed
Summary
This summary is machine-generated.

Ceramic workers show high Aspergillus (A.) presence in sputum via PCR. Elevated specific IgE (sIgE) for Aspergillus fumigatus indicates significant fungal exposure risks in this industry.

Related Experiment Videos

Last Updated: May 27, 2026

Collection and Extraction of Occupational Air Samples for Analysis of Fungal DNA
12:02

Collection and Extraction of Occupational Air Samples for Analysis of Fungal DNA

Published on: May 2, 2018

Area of Science:

  • Occupational Health
  • Mycology
  • Immunology

Background:

  • Ceramic workers face elevated risks of respiratory issues due to silica dust and microbial exposure.
  • High levels of Aspergillus (A.) species are frequently detected in environments with ceramic dust.
  • Understanding Aspergillus sensitization is crucial for assessing occupational health hazards.

Purpose of the Study:

  • To determine the prevalence of Aspergillus (A.) in ceramic workers using polymerase chain reaction (PCR).
  • To assess specific IgE (sIgE) levels against different Aspergillus species in ceramic workers.
  • To correlate Aspergillus (A.) detection and sensitization with occupational exposure.

Main Methods:

  • Polymerase chain reaction (PCR) was used to detect Aspergillus (A.) DNA in sputum samples.
  • Specific IgE (sIgE) levels for Aspergillus flavus, Aspergillus fumigatus, and Aspergillus niger were measured.
  • 40 ceramic workers and 56 control subjects were included in the study.

Main Results:

  • 32.5% of ceramic workers' sputum tested positive for Aspergillus (A.) via PCR.
  • Aspergillus flavus was the most common species detected (69.2%), followed by A. niger and A. fumigatus.
  • A significant increase (>100%) in sIgE for Aspergillus fumigatus was observed in workers compared to controls.

Conclusions:

  • Fungal exposure, particularly to Aspergillus (A.) species, poses a significant occupational hazard in the ceramic industry.
  • Elevated sIgE levels suggest sensitization to Aspergillus (A.) species in ceramic workers.
  • No significant correlation was found between the duration of exposure and sIgE levels for Aspergillus species.