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Related Concept Videos

Protein Organization01:24

Protein Organization

Proteins are polymers of amino acid residues. They are versatile and responsible for different cellular functions, including DNA replication, molecular transport, catalysis, and structural support. Proteins have a hierarchical structure comprising at least three levels of organization: primary, secondary, and tertiary structure. Some large proteins have a quaternary structure where individual protein subunits are linked together.
The primary structure of a protein is its amino acid sequence.
Protein Organization01:24

Protein Organization

Proteins are polymers of amino acid residues. They are versatile and responsible for different cellular functions, including DNA replication, molecular transport, catalysis, and structural support. Proteins have a hierarchical structure comprising at least three levels of organization: primary, secondary, and tertiary structure. Some large proteins have a quaternary structure where individual protein subunits are linked together.
The primary structure of a protein is its amino acid sequence.
Protein Organization01:13

Protein Organization

Overview
Protein Families02:47

Protein Families

Protein families are groups of homologous proteins; that is, they have similarities in amino acid sequences and three-dimensional structures. Protein families usually occur because of gene duplication, where an additional copy of a gene is inserted into the genome of an organism.   Mutations that change the amino acids but still allow the protein to be properly synthesized, will lead to new protein family members.   If these new proteins contain similar amino acids in key locations, protein...
Protein-protein Interfaces02:04

Protein-protein Interfaces

Many proteins form complexes to carry out their functions, making protein-protein interactions (PPIs) essential for an organism's survival. Most PPIs are stabilized by numerous weak noncovalent chemical forces. The physical shape of the interfaces determines the way two proteins interact. Many globular proteins have closely-matching shapes on their surfaces, which form a large number of weak bonds. Additionally, many PPIs occur between two helices or between a surface cleft and a polypeptide...
Protein and Protein Structure02:15

Protein and Protein Structure

Proteins are one of the most abundant organic molecules in living systems and have the most diverse range of functions of all macromolecules. Proteins may be structural, regulatory, contractile, or protective. They may serve in transport, storage, or membranes; or they may be toxins or enzymes. Their structures, like their functions, vary greatly. They are all, however, amino acid polymers arranged in a linear sequence.
A protein's shape is critical to its function. For example, an enzyme can...

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Updated: May 27, 2026

A Protocol for Computer-Based Protein Structure and Function Prediction
16:41

A Protocol for Computer-Based Protein Structure and Function Prediction

Published on: November 3, 2011

A protocol for computer-based protein structure and function prediction.

Ambrish Roy1, Dong Xu, Jonathan Poisson

  • 1Center for Computational Medicine and Bioinformatics, University of Michigan, USA.

Journal of Visualized Experiments : Jove
|November 16, 2011
PubMed
Summary
This summary is machine-generated.

The I-TASSER server provides computational protein structure and function predictions for millions of uncharacterized proteins. It offers high-resolution models and functional annotations, aiding biological understanding and research.

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Computational Prediction of Amino Acid Preferences of Potentially Multispecific Peptide-Binding Domains Involved in Protein-Protein Interactions
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A Protocol for Computer-Based Protein Structure and Function Prediction
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Application of I TASSER, trRosetta, UCSF Chimera, HADDOCK server, and HEX loria for De Novo and In Silico Design of Proteins
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Computational Prediction of Amino Acid Preferences of Potentially Multispecific Peptide-Binding Domains Involved in Protein-Protein Interactions
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Computational Prediction of Amino Acid Preferences of Potentially Multispecific Peptide-Binding Domains Involved in Protein-Protein Interactions

Published on: January 26, 2024

Area of Science:

  • Computational biology
  • Structural bioinformatics
  • Genomics

Background:

  • Genome sequencing yields millions of protein sequences needing structure and function determination.
  • Experimental methods are limited to a small protein fraction, necessitating computational approaches for uncharacterized proteins.

Purpose of the Study:

  • To present the I-TASSER server, an online platform for high-resolution protein structure and function modeling.
  • To provide accurate predictions for uncharacterized proteins, aiding biological role elucidation.

Main Methods:

  • Utilizes protein threading and structure alignment to detect homologous templates.
  • Generates full-length tertiary structural models and predicts functional annotations.
  • Incorporates user-specified constraints for interactive model refinement.

Main Results:

  • I-TASSER provides predictions with confidence scores, including secondary structure, solvent accessibility, and functional annotations (enzyme classification, GO terms, binding sites).
  • The server was ranked as the best for protein structure and function prediction in CASP experiments.
  • Over 20,000 scientists globally utilize the I-TASSER server.

Conclusions:

  • I-TASSER is a valuable computational tool for predicting protein structure and function.
  • Its accuracy and user-friendly interface facilitate biological research on a large scale.
  • The server empowers scientists to enhance protein structure predictions with experimental data or biological insights.