Physiological characterization and functional heterogeneity of narrow-field mammalian amacrine cells

Light-evoked responses of 106 morphologically identified narrow-field amacrine cells (ACs) were studied in dark-adapted mouse retinal slices. Forty-five cells exhibit AIIAC morphology, 55% of which show characteristic AIIAC physiological properties (AIIAC1s) and the remaining 45% display different physiological responses, suggesting that AIIACs are functionally heterogeneous. Moreover, we found that 42 cells exhibit morphology that resembles the seven morphological types of glycine-positive ACs (GlyAC1-7) reported in the rat retina, and for the first time assigned light response and function properties to these morphological types of glycinergic ACs in the mouse retina. In addition, five narrow-field ACs exhibited morphology resembling that of the GlyAC5 or GlyAC7 but with different physiological responses (GlyAC5(#) and GlyAC7(#)). Therefore, the eight morphological types of narrow-field ACs exhibit 12 classes of physiological responses. Furthermore, we found ACs whose physiological responses were indistinguishable from those of GlyAC3 or GlyAC4s but with different morphology (GlyAC3* or GlyAC4*). These observations suggest that although the majority of narrow-field mammalian ACs forms discrete functional groups that correlate with their morphology, a significant number of these cells with similar morphology do not display the same light responses, and some with similar light responses do not exhibit the same morphology.