Related Experiment Video
Updated: May 27, 2026

A High-Throughput Electrochemiluminescence 7-Plex Assay Simultaneously Screening for Type 1 Diabetes and Multiple Autoimmune Diseases
Published on: May 29, 2020
Multiplexed immunoassay of thyroglobulin autoantibodies in patients with differentiated thyroid carcinoma
Luca Giovanella1, Emanuela Toffalori, Renato Tozzoli
1Department of Nuclear Medicine and Thyroid Centre, Oncology Institute of Southern Switzerland, CH-6500 Bellinzona, Switzerland. luca.giovanella@eoc.ch
Background:
Thyroglobulin (Tg) antibodies (TgAb) screening is recommended in patients with differentiated thyroid carcinoma (DTC) to validate Tg measurements. We compared a new multiplexed and 2 conventional TgAb immunoassays in patients with DTC and healthy controls.
Methods:
TgAb were measured by 2 conventional automated immunoassays and a multiplexed immunoassay in sera from 163 patients with DTC and 64 controls.
Results:
No significant differences were found between the different assays when the suggested manufacturer cutoffs were used. The positive rate of multiplexed assay, but not of conventional assays, significantly increased when limits of detection were used as cutoff.
Conclusion:
Further technical evaluations are necessary to explain the very high rate of detectable TgAb values in both patients with DTC and controls by the multiplexed assay. For the moment, we suggest caution in using such methods in the current clinical practice.
Related Concept Videos
Hyperthyroidism II: Pathophysiology
Graves Disease II: Pathophysiology
Graves' Disease I: Introduction
Hyperthyroidism I: Introduction
Synthesis and Regulation of Thyroid Hormones
Upon reaching the thyroid gland, TSH stimulates the follicular cells' active uptake of iodide ions from the blood. The ions diffuse to the apical surface of the cells and are oxidized to iodine. The iodine is then...

