Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Synthetic Biology02:55

Synthetic Biology

Synthetic biology is an interdisciplinary science that involves using principles from disciplines such as engineering, molecular biology, cell biology, and systems biology. It involves remodeling existing organisms from nature or constructing completely new synthetic organisms for applications such as protein or enzyme production, bioremediation, value-added macromolecule production, and the addition of desirable traits to crops, to name a few.
Golden rice
Golden rice is a genetically modified...
Protein Networks02:26

Protein Networks

An organism can have thousands of different proteins, and these proteins must cooperate to ensure the health of an organism. Proteins bind to other proteins and form complexes to carry out their functions. Many proteins interact with multiple other proteins creating a complex network of protein interactions.
These interactions can be represented through maps depicting protein-protein interaction networks, represented as nodes and edges. Nodes are circles that are representative of a protein,...
Protein Networks02:26

Protein Networks

An organism can have thousands of different proteins, and these proteins must cooperate to ensure the health of an organism. Proteins bind to other proteins and form complexes to carry out their functions. Many proteins interact with multiple other proteins creating a complex network of protein interactions.
These interactions can be represented through maps depicting protein-protein interaction networks, represented as nodes and edges. Nodes are circles that are representative of a protein,...
Combinatorial Gene Control02:33

Combinatorial Gene Control

Combinatorial gene control is the synergistic action of several transcriptional factors to regulate the expression of a single gene. The absence of one or more of these factors may lead to a significant difference in the level of gene expression or repression.
The expression of more than 30,000 genes is controlled by approximately 2000-3000 transcription factors. This is possible because a single transcription factor can recognize more than one regulatory sequence. The specificity in gene...
Sequence Networks of Rotating Machines01:24

Sequence Networks of Rotating Machines

A Y-connected synchronous generator, grounded through a neutral impedance, is designed to produce balanced internal phase voltages with only positive-sequence components. The generator's sequence networks include a source voltage that is exclusively in the positive-sequence network. The sequence components of line-to-ground voltages at the generator terminals illustrate this configuration.
Zero-sequence current induces a voltage drop across the generator's neutral impedance and other...
Reporter Genes02:11

Reporter Genes

Reporter genes are a type of protein-coding gene that are often tagged to a gene of interest. Once inside a target cell, reporter genes usually produce visually identifiable characteristics like fluorescence and luminescence when expressed along with the gene of interest. Thus, reporter genes “report” the presence or absence of genes of interest in an organism, determine the gene expression pattern, or track the physical location of a DNA segment or protein in the cell.
Commonly used reporter...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Retina-specific long non-coding RNAs associated with inherited retinal disease genes.

Cellular and molecular life sciences : CMLS·2026
Same author

Engineering a PD-L1-sensing synthetic receptor for programmable macrophage response.

Journal of biological engineering·2026
Same author

Flux sampling and graph neural networks for improved gene essentiality prediction in mammalian genome-scale metabolic models.

NPJ systems biology and applications·2026
Same author

Systematic Evaluation of Different Ribonucleoprotein Complexes as Posttranscriptional Biosensors in Cell-Free TX-TL Systems.

ACS synthetic biology·2026
Same author

Knowledge preservation in the era of big science and AI: strategies for sustainable scientific research.

Nature communications·2026
Same author

Telethon Undiagnosed Disease Program: Structured approach to solving rare childhood-onset genetic diseases.

Genetics in medicine open·2026

Related Experiment Video

Updated: May 27, 2026

A Multilayer Microfluidic Platform for the Conduction of Prolonged Cell-Free Gene Expression
11:23

A Multilayer Microfluidic Platform for the Conduction of Prolonged Cell-Free Gene Expression

Published on: October 6, 2019

Predicting synthetic gene networks.

Diego di Bernardo1, Lucia Marucci, Filippo Menolascina

  • 1Faculty of Engineering, University of Naples "Fecerico II", Naples, Italy. dibernard@tigem.it

Methods in Molecular Biology (Clifton, N.J.)
|November 16, 2011
PubMed
Summary

Synthetic biology utilizes quantitative models to design and predict synthetic gene networks. This approach aids in understanding and engineering cellular functions in organisms like yeast and human cells.

More Related Videos

Mapping Bacterial Functional Networks and Pathways in Escherichia Coli using Synthetic Genetic Arrays
14:06

Mapping Bacterial Functional Networks and Pathways in Escherichia Coli using Synthetic Genetic Arrays

Published on: November 12, 2012

Automated Robotic Liquid Handling Assembly of Modular DNA Devices
11:22

Automated Robotic Liquid Handling Assembly of Modular DNA Devices

Published on: December 1, 2017

Related Experiment Videos

Last Updated: May 27, 2026

A Multilayer Microfluidic Platform for the Conduction of Prolonged Cell-Free Gene Expression
11:23

A Multilayer Microfluidic Platform for the Conduction of Prolonged Cell-Free Gene Expression

Published on: October 6, 2019

Mapping Bacterial Functional Networks and Pathways in Escherichia Coli using Synthetic Genetic Arrays
14:06

Mapping Bacterial Functional Networks and Pathways in Escherichia Coli using Synthetic Genetic Arrays

Published on: November 12, 2012

Automated Robotic Liquid Handling Assembly of Modular DNA Devices
11:22

Automated Robotic Liquid Handling Assembly of Modular DNA Devices

Published on: December 1, 2017

Area of Science:

  • Synthetic biology
  • Systems biology
  • Computational biology

Background:

  • Cellular regulation involves complex interactions.
  • Quantitative mathematical models are essential for understanding biological systems.
  • Synthetic biology designs novel biological functions.

Purpose of the Study:

  • To describe methods for modeling and predicting synthetic network behavior.
  • To apply these methods to two distinct biological systems.
  • To facilitate the design and construction of synthetic biological circuits.

Main Methods:

  • Development of quantitative mathematical models for cellular regulation.
  • Analysis of synthetic gene networks in Saccharomyces cerevisiae.
  • Modeling of a synthetic transcriptional positive feedback loop in HEK293 cells.

Main Results:

  • Successful modeling of a five-gene regulatory network in yeast.
  • Prediction of behavior for a synthetic transcriptional positive feedback loop in HEK293 cells.
  • Demonstration of the utility of mathematical modeling in synthetic biology.

Conclusions:

  • Quantitative models are crucial for designing and predicting synthetic biological systems.
  • The described modeling approaches are applicable across different organisms and network architectures.
  • This work advances the engineering of biological functions through synthetic biology.