Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Site-Targeted Drug Delivery Systems: Polymeric Carriers01:24

Site-Targeted Drug Delivery Systems: Polymeric Carriers

Polymeric carriers enhance targeted drug delivery by increasing efficacy while minimizing off-target effects. These carriers comprise a biodegradable polymeric backbone integrated with functional elements that enable targeting, improve physicochemical properties, and regulate drug release.Targeting MechanismsThe targeting ability of polymeric carriers is mediated by a homing device, which is a molecular recognition component designed to selectively bind to specific tissues or cells. Monoclonal...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Matrix Stiffness Induces Endothelial Network Senescence.

Advanced science (Weinheim, Baden-Wurttemberg, Germany)·2026
Same author

Derivation of functional retinal endothelial cells from human pluripotent stem cells for therapeutics and modelling.

Nature biomedical engineering·2026
Same author

PathwayEmbed: a computational tool to quantify intracellular signaling transduction states from transcriptomic data.

Bioinformatics (Oxford, England)·2026
Same author

Mineral-Derived Nanozymes Modulate Plant Redox Homeostasis and Nitrogen Metabolism to Improve Crop Productivity.

ACS nano·2026
Same author

Cross-Organ Toxicity and Metabolic Responses to Food Chain-Transferred Nanoplastics: Mechanistic Insights from a Multiomics Perspective.

ACS nano·2026
Same author

γδ T cell-stromal networks modulate matrix composition and vascularity in foreign body response.

Nature communications·2026

Related Experiment Video

Updated: May 27, 2026

Preparation of Thermoresponsive Nanostructured Surfaces for Tissue Engineering
12:22

Preparation of Thermoresponsive Nanostructured Surfaces for Tissue Engineering

Published on: March 1, 2016

Development of a biodegradable, temperature-sensitive dextran-based polymer as a cell-detaching substrate.

Guoming Sun1, Sravanti Kusuma, Sharon Gerecht

  • 1Department of Chemical and Biomolecular Engineering, Johns Hopkins University, Baltimore, MD 21218, USA.

Macromolecular Bioscience
|November 16, 2011
PubMed
Summary

A new biodegradable polymer (TSDAIE) allows easy, non-damaging detachment of endothelial progenitor cells (EPCs) for regenerative medicine. This temperature-sensitive material offers a promising alternative for cell culture and tissue engineering applications.

More Related Videos

Fabricating Degradable Thermoresponsive Hydrogels on Multiple Length Scales via Reactive Extrusion, Microfluidics, Self-assembly, and Electrospinning
12:07

Fabricating Degradable Thermoresponsive Hydrogels on Multiple Length Scales via Reactive Extrusion, Microfluidics, Self-assembly, and Electrospinning

Published on: April 16, 2018

Manipulating Living Cells to Construct Stable 3D Cellular Assembly Without Artificial Scaffold
07:09

Manipulating Living Cells to Construct Stable 3D Cellular Assembly Without Artificial Scaffold

Published on: October 26, 2018

Related Experiment Videos

Last Updated: May 27, 2026

Preparation of Thermoresponsive Nanostructured Surfaces for Tissue Engineering
12:22

Preparation of Thermoresponsive Nanostructured Surfaces for Tissue Engineering

Published on: March 1, 2016

Fabricating Degradable Thermoresponsive Hydrogels on Multiple Length Scales via Reactive Extrusion, Microfluidics, Self-assembly, and Electrospinning
12:07

Fabricating Degradable Thermoresponsive Hydrogels on Multiple Length Scales via Reactive Extrusion, Microfluidics, Self-assembly, and Electrospinning

Published on: April 16, 2018

Manipulating Living Cells to Construct Stable 3D Cellular Assembly Without Artificial Scaffold
07:09

Manipulating Living Cells to Construct Stable 3D Cellular Assembly Without Artificial Scaffold

Published on: October 26, 2018

Area of Science:

  • Biomaterials Science
  • Cell Biology
  • Regenerative Medicine

Background:

  • Endothelial progenitor cells (EPCs) are crucial for vascular repair.
  • Current methods for EPC detachment can be enzymatic and potentially damaging.
  • Developing non-enzymatic, gentle cell detachment methods is essential for preserving cell function.

Purpose of the Study:

  • To develop and evaluate a biodegradable, temperature-sensitive polymer substrate (TSDAIE) for nonenzymatic EPC detachment.
  • To optimize TSDAIE concentration for effective EPC culture and detachment.
  • To assess the compatibility of TSDAIE with EPCs during culture.

Main Methods:

  • Synthesis and characterization of temperature-sensitive dextran-allyl isocyanate-ethylamine (TSDAIE).
  • Coating cell culture flasks with TSDAIE and type I collagen.
  • Investigating TSDAIE phase transition temperature (18–22°C).
  • Evaluating EPC attachment, spreading, proliferation, and phenotype on TSDAIE-coated surfaces.

Main Results:

  • TSDAIE exhibits a lower critical solution temperature, enabling EPC detachment at 4°C.
  • Optimized TSDAIE concentration was determined based on EPC attachment.
  • TSDAIE demonstrated compatibility with EPCs, supporting cell attachment, spreading, proliferation, and phenotype maintenance.
  • The polymer is biodegradable and temperature-sensitive.

Conclusions:

  • Biodegradable TSDAIE is a promising nonenzymatic substrate for culturing and detaching human EPCs.
  • TSDAIE facilitates gentle cell detachment, preserving cell viability and function.
  • This technology holds potential for vascular regenerative medicine and tissue engineering applications.