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Related Concept Videos

Urine Studies I: Urinalysis01:29

Urine Studies I: Urinalysis

Urinalysis is a widely used diagnostic test that analyzes urine's physical, chemical, and microscopic characteristics. Healthcare providers use it to detect and monitor various health conditions, including renal disease, urinary tract infections (UTIs), diabetes, and metabolic or systemic disorders.Components of UrinalysisUrinalysis consists of three primary components: physical, chemical, and microscopic examination. Each provides unique insights into the urine sample and, by extension, the...

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Related Experiment Video

Updated: May 27, 2026

Proteomic Profile of EPS-Urine through FASP Digestion and Data-Independent Analysis
14:48

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Published on: May 8, 2021

Profiling of urine using ProteinChip® technology.

Ronald L Woodbury1, Diane L Bankert McCarthy, Amanda L Bulman

  • 1Bio-Rad Laboratories, Malvern, PA, USA. Ronald.Woodbu@amedd.army.mil

Methods in Molecular Biology (Clifton, N.J.)
|November 16, 2011
PubMed
Summary
This summary is machine-generated.

Urine biomarker discovery is enhanced by Surface-Enhanced Laser Desorption/Ionization-Time Of Flight (SELDI-TOF) analysis. This method allows direct urine analysis or enrichment, increasing detected proteins for disease research.

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Area of Science:

  • Biochemistry
  • Proteomics
  • Clinical Diagnostics

Background:

  • Urine is a valuable, non-invasively collected biofluid for biomarker discovery.
  • Its low protein content facilitates the detection of disease-associated perturbations.
  • Standard mass spectrometry often requires extensive sample cleanup, complicating urine analysis.

Purpose of the Study:

  • To evaluate Surface-Enhanced Laser Desorption/Ionization-Time Of Flight (SELDI-TOF) analysis for urine biomarker discovery.
  • To assess the benefits of sample enrichment prior to SELDI-TOF analysis of urine.
  • To highlight the importance of controlled sample collection and storage for reproducible results.

Main Methods:

  • Analysis of neat urine samples using SELDI-TOF on ProteinChip arrays.
  • Comparison of direct urine analysis versus analysis following a protein enrichment step.
  • Evaluation of the impact of sample collection and storage variability.

Main Results:

  • SELDI-TOF analysis is suitable for urine due to its tolerance for high salt and urea concentrations.
  • A protein enrichment step more than doubled the number of detected proteins in urine samples.
  • Variability in urine volume and composition necessitates controlled collection and storage protocols.

Conclusions:

  • SELDI-TOF is a powerful technique for urine biomarker discovery, offering advantages over traditional methods.
  • Sample enrichment significantly enhances proteomic profiling of urine.
  • Standardized pre-analytical procedures are critical for reliable and reproducible clinical biomarker studies.