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Updated: May 27, 2026

Real-Time Polymerase Chain Reaction-Based Detection and Quantification of Hepatitis B Virus DNA
Published on: December 15, 2023
[Seroprevalence of viral hepatitis B in university students in Abancay, Peru]
Max Carlos Ramírez-Soto1, Milagros Huichi-Atamari, Elsa Gladys Aguilar-Ancori
1Facultad de Ciencias Biológicas, Universidad Nacional de San Antonio Abad del Cusco, Cusco, Perú. maxcrs22@gmail.com
Abstract:
To determine the prevalence of serological markers of viral hepatitis B in university students of the city of Abancay, we performed a cross-sectional study on 240 students from three universities, from January to October 2010. Informed consent was requested to every student, an epidemiological record was filled, and a venous blood sample was drawn to determine the presence of HBsAg, total anti - HBcAg, anti - HBe, HBeAg and IgM Anti - HBc by ELISA. A prevalence of 2.5% (six positive samples) was found for HBsAg and of 28.3% (68 positive samples) for anti - HbcAg antibodies. The male sex was associated with the presence of anti - HBcAg (OR = 2.0, 95% CI, 1.2 to 3.6). We did not found HBeAg or IgM anti - HBc, however, the 6 HBsAg carriers were anti - HBe positive. In conclusion hepatitis B infection is still a public health problem in Abancay, with a significant prevalence in university students.
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