Related Experiment Video
Updated: May 27, 2026
07:23
Describing a Transcription Factor Dependent Regulation of the MicroRNA Transcriptome
Published on: June 15, 2016
Cell type-dependent gene regulation by Staufen2 in conjunction with Upf1
Takashi Miki1, Yasunao Kamikawa, Sadamu Kurono
1Department of Frontier Bioscience, Graduate School of Frontier Biosciences, Osaka University, Yamadaoka, Suita, Osaka, Japan.
BMC Molecular Biology
|November 18, 2011
Summary
Staufen 2 (Stau2) binds RNA helicase Upf1, influencing mRNA fate. Stau2
Area of Science:
- Molecular Biology
- Neuroscience
- RNA Biology
Background:
- Staufen 2 (Stau2) is implicated in dendritic mRNA targeting in neurons, but its regulatory mechanisms remain unclear.
- Understanding Stau2's function is crucial for deciphering mRNA transport and regulation in neuronal development and function.
Purpose of the Study:
- To identify novel binding partners of Stau2 to elucidate its function in mRNA regulation.
- To investigate the interaction between Stau2 and RNA helicase Upf1 and its impact on gene expression.
Main Methods:
- Affinity purification of GST-tagged Stau2 to identify binding partners.
- In vitro binding assays to confirm Stau2-Upf1 interaction.
- Reporter gene assays in different cell types (HeLa and 293F) to assess Stau2's effect on protein and mRNA levels.
Main Results:
- Three RNA helicases, including Upf1, were identified as Stau2 binding partners.
- Stau2 directly binds Upf1 in an RNA-independent manner.
- Stau2 upregulates reporter gene expression and mRNA levels in 293F cells, dependent on Upf1 for protein upregulation but not mRNA upregulation.
Conclusions:
- Stau2, alone or with Upf1, differentially regulates mRNA fate.
- Stau2-mediated mRNA fate determination exhibits cell type-specific effects.
More Related Videos
11:33Investigating Interactions Between Histone Modifying Enzymes and Transcription Factors in vivo by Fluorescence Resonance Energy Transfer
Published on: October 14, 2022
09:58Mapping the Structure-Function Relationships of Disordered Oncogenic Transcription Factors Using Transcriptomic Analysis
Published on: June 27, 2020
Related Concept Videos
01:23Regulation of Expression at Multiple Steps
The gene expression in cells is regulated at different stages: (i) transcription, (ii) RNA processing, (iii) RNA localization, and (iv) translation. Transcriptional regulation is mediated by regulatory proteins such as transcription factors, activators, or repressors—these control gene expression by initiating or inhibiting the transcription of genes. Once a precursor or pre-mRNA is produced, it undergoes post-transcriptional modification, including 5' capping, splicing, and the addition of a...
02:24Regulation of Expression Occurs at Multiple Steps
Gene expression can be regulated at almost every step from gene to protein. Transcription is the step that is most commonly regulated. This involves the binding of proteins to short regulatory sequences on the DNA. This association can either promote or inhibit the transcription of a gene associated with the respective sequence.
Transcription results in the generation of precursor (pre-mRNA) that consists of both exons and introns, which needs further processing before being translated to a...
Transcription results in the generation of precursor (pre-mRNA) that consists of both exons and introns, which needs further processing before being translated to a...
02:24Regulation of Expression Occurs at Multiple Steps
Gene expression can be regulated at almost every step from gene to protein. Transcription is the step that is most commonly regulated. This involves the binding of proteins to short regulatory sequences on the DNA. This association can either promote or inhibit the transcription of a gene associated with the respective sequence.
Transcription results in the generation of precursor (pre-mRNA) that consists of both exons and introns, which needs further processing before being translated to a...
Transcription results in the generation of precursor (pre-mRNA) that consists of both exons and introns, which needs further processing before being translated to a...
01:30General Transcription Factors
Tissue-specific transcription factors contribute to diverse cellular functions in mammals. For example, the gene for beta globin, a major component of hemoglobin, is present in all cells of the body. However, it is only expressed in red blood cells because the transcription factors that can bind to the promoter sequences of the beta globin gene are only expressed in these cells. Tissue-specific transcription factors also ensure that mutations in these factors may impair only the function of...
02:33Combinatorial Gene Control
Combinatorial gene control is the synergistic action of several transcriptional factors to regulate the expression of a single gene. The absence of one or more of these factors may lead to a significant difference in the level of gene expression or repression.
The expression of more than 30,000 genes is controlled by approximately 2000-3000 transcription factors. This is possible because a single transcription factor can recognize more than one regulatory sequence. The specificity in gene...
The expression of more than 30,000 genes is controlled by approximately 2000-3000 transcription factors. This is possible because a single transcription factor can recognize more than one regulatory sequence. The specificity in gene...
02:23Master Transcription Regulators
Master transcription regulators are regulatory proteins that are predominantly responsible for regulating the expression of multiple genes. Often these genes work in concert to drive a complex process. Activation of a master transcription regulator can lead to a cascade of transcriptional activation necessary for that outcome. These regulators can directly bind to the regulatory sequences of the various genes involved, or they can indirectly regulate transcription by binding to regulatory...