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Updated: May 27, 2026

Extracellular Vesicle Tissue Factor Activity Assay
Published on: December 29, 2023
Effects of four commercially available factor Xa proteins on the fluorogenic anti-factor Xa assay when monitoring
Vanessa Castro-López1, Brian Murray, Leanne F Harris
1Biomedical Diagnostics Institute, National Centre for Sensor Research, Dublin City University, Dublin, Ireland.
Abstract:
Four commercially available factor Xa (FXa) reagents were evaluated in a fluorogenic anti-FXa assay. The four reagents - of which three were of human origin and the fourth was bovine - were compared in terms of the resulting assay dynamic ranges, lag times, coefficient of variation and R2 values, as well as their sensitivity to unfractionated heparin within the therapeutic range of 0-1.2 U/ml. Similar performance of reagents in the fluorogenic anti-FXa assay was observed independent of the source of the reagent or its physical state, which may assist in the standardization of coagulation assays in clinical settings.
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