Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Accessing chiral NHC-boranes by abstraction of C<sub>6</sub>F<sub>5</sub> from the B(C<sub>6</sub>F<sub>5</sub>)<sub>4</sub><sup>-</sup> weakly coordinating anion.

Dalton transactions (Cambridge, England : 2003)·2026
Same author

Potent Biological Activity by a Synthetic Cu(I) Cationophore Redistributing Intracellular Copper Pools.

Journal of the American Chemical Society·2025
Same author

Unveiling the enzymatic activity of a dimeric LDH isoform and its implications for allosteric inhibition strategies.

Protein science : a publication of the Protein Society·2025
Same author

Cyanostilbenes as Selective Chloride Carriers Relying on CH-anion Interactions.

Chemistry (Weinheim an der Bergstrasse, Germany)·2025
Same author

Structural and Enzymological Characterization of Phosphoserine Phosphatase From Brucella melitensis.

Proteins·2025
Same author

Site-Specific Incorporation of Clickable d-Mannose Derivatives in the Lipopolysaccharide Core of the Pathogen <i>Brucella abortus</i>.

ACS chemical biology·2025

Related Experiment Video

Updated: May 27, 2026

Radiosynthesis of 1-(2-[18F]Fluoroethyl)-L-Tryptophan using a One-pot, Two-step Protocol
08:33

Radiosynthesis of 1-(2-[18F]Fluoroethyl)-L-Tryptophan using a One-pot, Two-step Protocol

Published on: September 21, 2021

4-Nitro-N-phthalyl-l-tryptophan.

Anaelle Tilborg, Irving Boittiaux, Bernadette Norberg

    Acta Crystallographica. Section E, Structure Reports Online
    |November 18, 2011
    PubMed
    Summary

    This study details the crystal structure of a novel compound, an analogue of epigenetic modulator RG108. Its structure is stabilized by hydrogen bonds and pi-pi stacking interactions between indole and phthalimide rings.

    Area of Science:

    • Crystallography
    • Structural Chemistry
    • Molecular Biology

    Background:

    • Epigenetic modifications play a crucial role in gene regulation.
    • RG108 is a known epigenetic modulator influencing DNA methylation.
    • Understanding the structural basis of such modulators is key to developing new therapeutic agents.

    Purpose of the Study:

    • To elucidate the crystal structure of a novel analogue of the epigenetic modulator RG108.
    • To identify the intermolecular interactions stabilizing the crystal lattice.
    • To provide insights into the structure-activity relationship of RG108 analogues.

    Main Methods:

    • Single-crystal X-ray diffraction was employed to determine the crystal structure.
    • Analysis of hydrogen bonding and π-π stacking interactions was performed.

    More Related Videos

    PCR Mutagenesis, Cloning, Expression, Fast Protein Purification Protocols and Crystallization of the Wild Type and Mutant Forms of Tryptophan Synthase
    09:31

    PCR Mutagenesis, Cloning, Expression, Fast Protein Purification Protocols and Crystallization of the Wild Type and Mutant Forms of Tryptophan Synthase

    Published on: September 26, 2020

    Chemical Modification of the Tryptophan Residue in a Recombinant Ca2+-ATPase N-domain for Studying Tryptophan-ANS FRET
    12:07

    Chemical Modification of the Tryptophan Residue in a Recombinant Ca2+-ATPase N-domain for Studying Tryptophan-ANS FRET

    Published on: October 9, 2021

    Related Experiment Videos

    Last Updated: May 27, 2026

    Radiosynthesis of 1-(2-[18F]Fluoroethyl)-L-Tryptophan using a One-pot, Two-step Protocol
    08:33

    Radiosynthesis of 1-(2-[18F]Fluoroethyl)-L-Tryptophan using a One-pot, Two-step Protocol

    Published on: September 21, 2021

    PCR Mutagenesis, Cloning, Expression, Fast Protein Purification Protocols and Crystallization of the Wild Type and Mutant Forms of Tryptophan Synthase
    09:31

    PCR Mutagenesis, Cloning, Expression, Fast Protein Purification Protocols and Crystallization of the Wild Type and Mutant Forms of Tryptophan Synthase

    Published on: September 26, 2020

    Chemical Modification of the Tryptophan Residue in a Recombinant Ca2+-ATPase N-domain for Studying Tryptophan-ANS FRET
    12:07

    Chemical Modification of the Tryptophan Residue in a Recombinant Ca2+-ATPase N-domain for Studying Tryptophan-ANS FRET

    Published on: October 9, 2021

  • The crystal structure was compared to known epigenetic modulators.

    • Main Results:

    • The crystal structure of (2R)-3-(1H-indol-3-yl)-2-(4-nitro-1,3-dioxoisoindolin-2-yl)propanoic acid (C19H13N3O6) was determined.
    • Strong intermolecular hydrogen bonds were observed between the indole N-H and phthalimide carbonyl oxygen, and between the carboxyl group and phthalimide carbonyl oxygen.
    • Significant π-π stacking interactions between indole and phthalimide rings were identified with centroid-centroid distances of 3.638(1) and 3.610(1) Å.

    Conclusions:

    • The crystal structure reveals specific hydrogen bonding and π-π stacking interactions that dictate the compound's conformation.
    • These interactions are crucial for the molecular recognition and potential biological activity of this RG108 analogue.
    • The findings contribute to the understanding of epigenetic modulator structures and inform future drug design.