Related Experiment Video
Updated: May 27, 2026

Genotyping of Staphylococcus aureus by Ribosomal Spacer PCR (RS-PCR)
Published on: November 4, 2016
The characterization and comparison of Staphylococcus aureus by antibiotic susceptibility testing, enterobacterial
Yingwang Ye1, Qihuan Jiang, Qingping Wu
1School of Biotechnology and Food Engineering, Hefei University of Technology, Hefei, China. yeyw04@mails.gucas.ac.cn
Abstract:
Thirty-five of Staphylococcus aureus isolated from food raw materials and workers' hands in a noshery were characterized using antibiotic susceptibility testing, enterobacterial repetitive intergenic consensus-polymerase chain reaction (ERIC-PCR), and random amplified polymorphic DNA (RAPD)-PCR. As a similarity threshold of 90%, ERIC-PCR classified 35 S. aureus isolates into 28 ERIC types with discrimination indexes (D) of 0.984, while RAPD-PCR grouped 35 S. aureus isolates into five clusters (A-E) showing 19 RAPD types with D of 0.949. Four resistance patterns were observed with D of 0.826. A comparison of characterization of S. aureus indicated a clear correlation between ERIC-PCR and RAPD-PCR or resistance patterns in some strains. It was concluded that ERIC-PCR method could be used for genetic diversity of S. aureus and tracing the sources of it from the food chain.
Related Concept Videos
Mechanism of Antibiotic Resistance in MRSA
Clinical Significance of Antibiotic Resistance
Automated Microbial Diagnostics
Modern Molecular Taxonomy
Methods of Classification and Identification
Staphylococcal Skin Infections
