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Impact of solvent/detergent treatment of plasma on transfusion-relevant bacteria
1Department of Microbiology and Immunology, Taipei Medical University, Taipei, Taiwan.
Background:
A solvent/detergent (S/D) treatment in a medical device has been developed for pathogen reduction of plasma for transfusion. Impact of S/D on bacterial growth and on the capacity of complement to kill bacteria has been investigated in this study.
Study Design And Methods:
A pool of apheresis plasma from four donors was spiked with eight transfusion-relevant bacteria. Plasma was treated with 1% tri(n-butyl) phosphate and 1% Triton X-45 at 31°C for 90 min and then extracted by oil at 31°C for 70 min. Decomplemented plasma and Phosphate Buffer Saline were used as controls. Bacterial count was determined in samples taken immediately after spiking, or after S/D and oil treatment. Similar experiments were conducted using three individual recovered plasma donations. Bacteria growth inhibition tests were performed using discs soaked with plasma samples whether containing the S/D agents or not.
Results:
The mean reduction factors of Escherichia coli, Pseudomonas aeruginosa, and Klebsiella pneumoniae due to complement during S/D treatment were >8·75, 4·71, and 4·18 log in pooled plasma and >7·42, 2·24 and >6·08 log in individual plasmas, respectively. Bacillus cereus and Bacillus subtilis were inactivated by S/D (>7·04 and 1·60 log in pooled, and >6·06 and 2·39 in individual plasmas, respectively). Staphylococcus aureus, Staphylococcus epidermidis and Enterobacter cloacae did not multiply during S/D treatment of plasma. Growth inhibition tests revealed an inhibition of three gram-negative bacteria by complement and all gram-positive by S/D.
Conclusion:
The S/D treatment of plasma does not alter the bactericidal activity of complement, and inactivates some gram-positive bacteria.
Insights
Solvent/detergent (S/D) treatment effectively reduces bacteria in plasma without harming complement
Area of Science:
- Transfusion Medicine
- Microbiology
- Biotechnology
Background:
- Solvent/detergent (S/D) treatment is a method for pathogen reduction in plasma for transfusion.
- The impact of S/D treatment on bacterial growth and complement activity in plasma requires investigation.
Purpose of the Study:
- To evaluate the effect of S/D treatment on bacterial growth in plasma.
- To assess the impact of S/D treatment on the complement system's ability to kill bacteria.
Main Methods:
- Plasma was spiked with eight transfusion-relevant bacteria and treated with S/D agents.
- Bacterial counts were determined post-treatment.
- Bacterial growth inhibition assays were performed using S/D-treated and untreated plasma.
Main Results:
- S/D treatment inactivated Bacillus cereus and Bacillus subtilis.
- Complement-mediated reduction of Escherichia coli, Pseudomonas aeruginosa, and Klebsiella pneumoniae was observed.
- Staphylococcus aureus, Staphylococcus epidermidis, and Enterobacter cloacae did not multiply during S/D treatment.
Conclusions:
- S/D treatment of plasma does not impair complement's bactericidal activity.
- S/D treatment effectively inactivates certain gram-positive bacteria, contributing to pathogen reduction in plasma.
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