Reference intervals for Greyhounds and Lurchers using the Sysmex XT-2000iV hematology analyzer

Area of Science:

• Veterinary Hematology
• Canine Clinical Pathology

Background:

• Breed-specific reference intervals (RIs) are needed for Greyhounds.
• Lurchers, as sighthound cross-breeds, may also require distinct hematologic RIs.
• Established RIs for Greyhounds and Lurchers using the Sysmex XT-2000iV hematology analyzer are lacking.

Purpose of the Study:

• Establish Greyhound RIs using the Sysmex XT-2000iV.
• Assess the transferability of Greyhound and non-sighthound RIs to Lurchers.
• Generate new Lurcher RIs if transference is not feasible.

Main Methods:

• Retrospective analysis of blood donor dog data.
• Nonparametric methods for Greyhound RI establishment (n=179).
• RI transference study for Lurchers (n=38) following CLSI guidelines; robust method used for new RI generation if needed.

Main Results:

• Greyhound RIs confirmed breed-specific hematologic profiles (higher RBC mass, lower WBC/platelets).
• Hemoglobin, HCT, MCV, MCH, MCHC, WBC, neutrophil, lymphocyte, monocyte, and platelet RIs were transferable from Greyhounds to Lurchers.
• New RIs were necessary for red blood cell (RBC) and eosinophil counts in Lurchers.

Conclusions:

• Lurchers exhibit significant hematologic similarities to Greyhounds.
• Distinct reference limits for RBC and eosinophil counts are indicated for Lurchers.
• This research provides essential breed-specific hematologic data for Greyhounds and Lurchers.