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Serum resistance in Haemophilus parasuis SC096 strain requires outer membrane protein P2 expression

Bin Zhang1, Saixiang Feng, Chenggang Xu

  • 1Key Laboratory of Veterinary Vaccine Innovation of the Ministry of Agriculture, College of Veterinary Medicine, South China Agricultural University, Guangzhou, China.

FEMS Microbiology Letters
|November 19, 2011
PubMed

Insights

Haemophilus parasuis outer membrane protein P2 (OmpP2) is crucial for bacterial survival. Deleting OmpP2 in the SC096 strain increased susceptibility to serum, highlighting its role in serum resistance.

Area of Science:

  • Bacteriology
  • Immunology
  • Molecular Biology

Background:

  • Haemophilus parasuis outer membrane protein P2 (OmpP2) is abundant and antigenic.
  • OmpP2 is a potential virulence factor in H. parasuis infections.

Purpose of the Study:

  • To investigate the precise function of OmpP2 in H. parasuis.
  • To determine OmpP2's role in bacterial growth and serum resistance.

Main Methods:

  • Construction of an ompP2-deficient mutant (ΔompP2) using a modified natural transformation system.
  • Comparison of growth and serum sensitivity between wild-type, ΔompP2 mutant, and complemented H. parasuis strains.

Main Results:

  • The ΔompP2 mutant exhibited a significant growth defect compared to the wild-type.
  • The ΔompP2 mutant showed increased sensitivity to the bactericidal effects of porcine and rabbit sera.
  • Complementation of the mutant strain restored normal growth and serum resistance phenotypes.

Conclusions:

  • H. parasuis OmpP2 is an important surface protein involved in serum resistance.
  • OmpP2 plays a significant role in the survival of H. parasuis SC096 in the presence of serum.

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