Related Experiment Video
Updated: May 27, 2026

Identification of Rare Bacterial Pathogens by 16S rRNA Gene Sequencing and MALDI-TOF MS
Published on: July 11, 2016
Stenotrophomonas interspecies differentiation and identification by gyrB sequence analysis
Liselott A Svensson-Stadler1, Sashka A Mihaylova, Edward R B Moore
1Culture Collection University of Gothenburg (CCUG), Department of Infectious Disease, Sahlgrenska Academy of the University of Gothenburg, Gothenburg, Sweden. iselott@ccug.se
Distinguishing Stenotrophomonas species is challenging with traditional methods. Analyzing the gyrB gene offers a more reliable way to identify Stenotrophomonas species, revealing potential new species within S. maltophilia.
Area of Science:
- Microbiology
- Genetics
- Bacterial Taxonomy
Background:
- Stenotrophomonas species are prevalent in diverse environments and clinical settings.
- Stenotrophomonas maltophilia is a significant opportunistic human pathogen.
- Current phenotyping and 16S rRNA gene sequencing methods struggle to reliably differentiate Stenotrophomonas species.
Purpose of the Study:
- To develop a more accurate method for distinguishing Stenotrophomonas species.
- To investigate the genetic diversity within the S. maltophilia complex.
Main Methods:
- Sequence analysis of two targeted regions of the gyrB gene (encoding the β-subunit of DNA gyrase).
- Polymerase Chain Reaction (PCR) amplification of specific gyrB gene fragments.
- Comparison of gyrB gene sequence dissimilarities among Stenotrophomonas type strains and clinical isolates.
Main Results:
- The gyrB gene sequencing effectively resolved and identified different Stenotrophomonas species, showing >7% dissimilarity between species.
- Strains within the same species exhibited up to 4.6% and 5.9% dissimilarity in the two gyrB regions analyzed.
- Strains identified as S. maltophilia, despite high 16S rRNA gene similarity (>99.0%), showed low gyrB similarities (as low as 93%) and <70% genomic DNA similarity to the type strain, suggesting distinct species.
Conclusions:
- GyrB gene sequence analysis provides a robust tool for Stenotrophomonas species identification.
- The 'S. maltophilia complex' likely encompasses multiple distinct species.
- Further taxonomic studies are warranted to reclassify strains currently identified as S. maltophilia.
More Related Videos
10:03Isolation, Propagation, and Identification of Bacterial Species with Hydrocarbon Metabolizing Properties from Aquatic Habitats
Published on: December 7, 2021
09:43Subtyping of Campylobacter jejuni ssp. doylei Isolates Using Mass Spectrometry-based PhyloProteomics (MSPP)
Published on: October 30, 2016
Related Concept Videos
Modern Molecular Taxonomy
Methods of Classification and Identification
Applications of Molecular Taxonomy
Phylogenetic Species Concept in Microbiology