Related Experiment Video
Updated: May 27, 2026
09:34
Scratch Migration Assay and Dorsal Skinfold Chamber for In Vitro and In Vivo Analysis of Wound Healing
Published on: September 26, 2019
CEACAM1 deficiency delays important wound healing processes.
Sarah LeBlanc1, Azadeh Arabzadeh, Samantha Benlolo
1Rosalind and Morris Goodman Cancer Research Centre, McGill University, Montreal, Quebec, Canada.
Summary
Carcinoembryonic antigen-related cell adhesion molecule 1 (CEACAM1) deletion impairs cutaneous wound healing. CEACAM1 deficiency reduces macrophage infiltration, delays reepithelialization, and diminishes vascularization in healing skin wounds.
Area of Science:
- Dermatology
- Immunology
- Cell Biology
Background:
- Cutaneous wound healing involves complex interactions between multiple cell types.
- Carcinoembryonic antigen-related cell adhesion molecule 1 (CEACAM1) plays roles in angiogenesis, cell migration, and immune functions.
- CEACAM1 is expressed in various cell types relevant to tissue repair.
Purpose of the Study:
- To investigate the role of CEACAM1 in cutaneous wound healing.
- To determine if CEACAM1 deficiency impacts key processes of wound repair.
Main Methods:
- Induction of standardized skin wounds in wild-type and Ceacam1(-/-) mice.
- Analysis of inflammatory cell infiltration (macrophages).
- Assessment of reepithelialization rates and granulation tissue vascular density.
Main Results:
- CEACAM1 deletion led to decreased F4/80(+) macrophage infiltration, indicating altered inflammatory responses.
- Reepithelialization was significantly delayed in Ceacam1(-/-) wounds compared to controls.
- Granulation tissue in Ceacam1(-/-) wounds exhibited significantly reduced vascular density.
Conclusions:
- CEACAM1 is a crucial regulator of cutaneous wound healing.
- CEACAM1 influences inflammatory cell recruitment, epithelial closure, and angiogenesis during wound repair.
- Further research is needed to elucidate whether CEACAM1 acts through specific cell types or multiple pathways.
Related Concept Videos
01:31Immunoglobulin-like Cell Adhesion Molecules
Immunoglobulin-like cell adhesion molecules or Ig-CAMs are a versatile group of cell surface glycoproteins belonging to the immunoglobulin protein superfamily. Ig-CAMs possess the characteristic immunoglobulin protein domains and other domains such as the fibronectin type III domain. The Ig domains are glycosylated to varying degrees in different Ig-CAMs.
Ig-CAMs exhibit either homophilic binding (to other Ig-CAMs) or heterophilic binding (to other ligands such as integrins). While most Ig-CAMs...
Ig-CAMs exhibit either homophilic binding (to other Ig-CAMs) or heterophilic binding (to other ligands such as integrins). While most Ig-CAMs...
01:24Healing II: Complications
Complications during healing arise when tissue repair is altered by local or systemic factors. These changes involve abnormal collagen deposition, altered biomechanics, and reduced vascular supply, impairing restoration of normal structure and function.Loss of FunctionScar tissue differs significantly from the original tissue it replaces. In the skin, fibrosis lacks adnexal structures such as hair follicles, sebaceous glands, and sweat glands. Their absence reduces tactile sensitivity, impairs...
01:20Cell Adhesion Molecules - Types and Functions
Cell adhesion molecules (CAMs) are pivotal to multicellularity and the coordinated functioning of tissues and organ systems. They enable physical interactions between cells and provide mechanical strength to tissues. They also function as receptors for signal transmission across the plasma membrane. The CAMs are broadly classified into four families - integrins, cadherins, selectins, and immunoglobulin-like CAMs (IgCAMs).
CAM Families
The Integrin family of proteins is primarily involved in a...
CAM Families
The Integrin family of proteins is primarily involved in a...
01:19Clinical Applications of Epidermal Stem Cells
Epidermal stem cells (EpiSCs) are mainly located at the basal layer of the epidermis. These cells repair minor injuries of the skin and replace dead skin cells. However, EpiSCs’ cannot heal severe wounds such as major burns or those from diabetes or hereditary disorders. In such cases, culturing the epidermal stem cells from the patient is possible and has yielded successful treatment options, such as laboratory-grown skin grafts. These grafts are synthesized using a patient’s own EpiSCs...
01:12Role Of Notch Signalling In Intestinal Stem Cell Renewal
Notch signaling was first discovered in Drosophila melanogaster, where it is involved in cell lineage differentiation. Notch signaling regulates the maintenance and differentiation of intestinal stem cells or ISCs by controlling the expression of atonal homolog 1 or Atoh1. Atoh1 directs cells to differentiate into secretory cells.
Direct cell-to-cell contact is needed for the activation of Notch signaling. The signal is initiated when a notch ligand binds to a receptor on an adjacent cell, also...
Direct cell-to-cell contact is needed for the activation of Notch signaling. The signal is initiated when a notch ligand binds to a receptor on an adjacent cell, also...
01:22Role of Ephrin-Eph Signalling in Intestinal Stem Cell Renewal
Erythropoietin-producing hepatocellular carcinoma receptor (Eph) and its ligand, Eph receptor-interacting protein (Ephrin) were first discovered in the human carcinoma cell line, hence the name. Ephrin-Eph interaction guides cells to reach their appropriate location in adult tissues. They also play an essential role in the immune system by helping in immune cell migration, adhesion, and activation. Based on their structure and function, Eph is divided into two classes — EphA and EphB.